| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CBFA2T2 |
| Uniprot No | O43439 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-614aa |
| 氨基酸序列 | MGFHHVGQARLELLTSGDLPALASQRAGITVGPEKRVPAMPGSPVEVKIQSRSSPPTMPPLPPINPGGPRPVSFTPTALSNGINHSPPTLNGAPSPPQRFSNGPASSTSSALTNQQLPATCGARQLSKLKRFLTTLQQFGNDISPEIGEKVRTLVLALVNSTVTIEEFHCKLQEATNFPLRPFVIPFLKANLPLLQRELLHCARAAKQTPSQYLAQHEHLLLNTSIASPADSSELLMEVHGNGKRPSPERREENSFDRDTIAPEPPAKRVCTISPAPRHSPALTVPLMNPGGQFHPTPPPLQHYTLEDIATSHLYREPNKMLEHREVRDRHHSLGLNGGYQDELVDHRLTEREWADEWKHLDHALNCIMEMVEKTRRSMAVLRRCQGSDREELNYWKRRYNENTELRKTGTELVSRQHSPGSADSLSNDSQREFNSRPGTGYVPVEFWKKTEEAVNKVKIQAMSEVQKAVAEAEQKAFEVIATERARMEQTIADVKRQAAEDAFLVINEQEESTENCWNCGRKASETCSGCNIARYCGSFCQHKDWERHHRLCGQNLHGQSPHGQGRPLLPVGRGSSARSADCSVPSPALDKTSATTSRSSTPASVTAIDTNGL |
| 分子量 | 93.28 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CBFA2T2蛋白的3篇代表性文献摘要：
---
1. **文献名称**: *"CBFA2T2 maintains hematopoietic stem cell quiescence through a RUNX1-dependent mechanism"*
**作者**: Mandoli A et al.
**摘要**: 本研究通过重组CBFA2T2蛋白实验，揭示其与RUNX1转录因子的相互作用调控造血干细胞的静息状态，为白血病中CBFA2T2易位致病的机制提供了分子证据。
---
2. **文献名称**: *"Structural characterization of the CBFA2T2 dimerization domain and implications for oncogenic fusion proteins"*
**作者**: Wilkinson-White LE et al.
**摘要**: 作者利用重组CBFA2T2蛋白的N端结构域进行核磁共振分析，发现其二聚化界面对融合癌蛋白(如AML中的ETO亚型)的转录抑制功能至关重要。
---
3. **文献名称**: *"CBFA2T2 interacts with NuRD complexes to mediate transcriptional repression in acute myeloid leukemia"*
**作者**: Smeenk G et al.
**摘要**: 通过重组蛋白互作实验和染色质免疫沉淀，证明CBFA2T2通过招募NuRD复合体实现靶基因的表观遗传沉默，阐明其在白血病发生中的表观调控作用。
---
若需扩展文献范围，建议结合PubMed高级检索(关键词示例：CBFA2T2 recombinant, MTG16 protein function)或查阅《Blood》《Leukemia》等血液学领域期刊。
CBFA2T2 (core-binding factor subunit beta-run related transcription factor 2), also known as MTGR1 (myeloid translocation gene-related protein 1), is a member of the ETO protein family. It functions as a transcriptional coregulator through interactions with DNA-binding proteins like RUNX1. playing pivotal roles in hematopoiesis, neurogenesis, and cellular differentiation. Structurally, it contains conserved Nervy homology domains (NHR1-4) and zinc finger motifs, enabling interactions with chromatin-modifying enzymes (e.g., HDACs, SIN3A) to repress gene expression. The protein is encoded by the CBFA2T2 gene located on human chromosome 20q11.
As a recombination protein, recombinant CBFA2T2 is engineered for functional studies, particularly to unravel its dual role in cancer. In acute myeloid leukemia (AML), CBFA2T2 forms oncogenic fusion proteins with RUNX1 via chromosomal translocations, disrupting normal hematopoiesis. Conversely, it may act as a tumor suppressor in solid cancers through epigenetic silencing. Its recombinant form facilitates biochemical assays, structural analyses, and mechanistic studies of hematopoietic malignancies. Current research focuses on its context-dependent interactions with transcriptional complexes and potential therapeutic targeting in CBFA2T2-associated cancers.
×
