| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CATSPER3 |
| Uniprot No | Q86XQ3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 299-398aa |
| 氨基酸序列 | QRQQEEISRLMHIQKNADCTSFSELVENFKKTLSHTDPMVLDDFGTSLPFIDIYFSTLDYQDTTVHKLQELYYEIVHVLSLMLEDLPQEKPQSLEKVDEK |
| 分子量 | 36.74 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人阳离子通道精子相关蛋白3(CATSPER3)的参考文献示例：
1. **文献名称**: "CATSPER3: A key component of sperm-specific cation channel complexes"
**作者**: Lobley A. et al.
**摘要**: 本研究通过免疫共沉淀和基因敲除模型证实，CATSPER3是精子阳离子通道复合体的核心组分，其缺失导致精子钙内流异常及男性不育。
2. **文献名称**: "Functional characterization of CATSPER3 in sperm motility regulation"
**作者**: Avidan N. et al.
**摘要**: 研究者利用小鼠模型发现，CATSPER3基因特异性表达于睾丸组织，敲除后精子运动能力显著下降，提示其在超活化运动中的关键作用。
3. **文献名称**: "Structural insights into CATSPER channel assembly through cryo-EM"
**作者**: Chung J.J. et al.
**摘要**: 通过冷冻电镜解析CATSPER复合体结构，揭示CATSPER3与其他亚基的互作模式，阐述其在通道门控及钙信号传导中的分子机制。
4. **文献名称**: "Gene polymorphisms of CATSPER3 correlate with idiopathic male infertility"
**作者**: Qi H. et al.
**摘要**: 临床研究发现，CATSPER3基因多态性与特发性男性不育显著相关，为男性生殖疾病提供了潜在分子标记。
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**注释**：以上文献名称和内容为综合领域研究的概括性示例，实际引用需以具体发表文章为准。建议通过PubMed或Web of Science检索关键词"CATSPER3"获取最新研究。
CATSPER3 (Cation Channel Sperm-Associated Protein 3) is a subunit of the CATSPER channel, a pH-sensitive, calcium-permeable ion channel critical for sperm function. Belonging to the voltage-gated calcium channel family, the CATSPER complex is uniquely expressed in sperm and comprises four pore-forming subunits (CATSPER1-4) alongside auxiliary proteins. CATSPER3 localizes to the principal piece of the sperm flagellum and plays a vital role in regulating calcium influx during sperm hyperactivation, a process essential for penetrating the egg’s protective layers.
This channel is activated by alkalization and physiological ligands (e.g., progesterone), triggering intracellular calcium surges that drive flagellar beating patterns and acrosome reaction. Male infertility studies link CATSPER3 mutations to impaired sperm motility and dysfunctional calcium signaling, often manifesting as asthenozoospermia. Knockout mouse models confirm its necessity for fertilization, showing sperm unable to navigate the female reproductive tract.
Research into CATSPER3 focuses on its structural interactions within the channel, calcium signaling mechanisms, and potential as a target for non-hormonal contraceptives. Its sperm-specific expression minimizes off-target effects in therapeutic interventions. However, detailed structural insights remain limited due to challenges in isolating intact channels. Advances in cryo-EM and heterologous expression systems are unraveling its activation pathways, offering promise for addressing male infertility and developing novel reproductive therapies. CATSPER3 thus represents a key player in reproductive biology, bridging calcium dynamics to functional fertility outcomes.
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