| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CASC4 |
| Uniprot No | Q6P4E1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-436aa |
| 氨基酸序列 | MVGFGANRRAGRLPSLVLVVLLVVIVVLAFNYWSISSRHVLLQEEVAELQGQVQRTEVARGRLEKRNSDLLLLVDTHKKQIDQKEADYGRLSSRLQAREGLGKRCEDDKVKLQNNISYQMADIHHLKEQLAELRQEFLRQEDQLQDYRKNNTYLVKRLEYESFQCGQQMKELRAQHEENIKKLADQFLEEQKQETQKIQSNDGKELDINNQVVPKNIPKVAENVADKNEEPSSNHIPHGKEQIKRGGDAGMPGIEENDLAKVDDLPPALRKPPISVSQHESHQAISHLPTGQPLSPNMPPDSHINHNGNPGTSKQNPSSPLQRLIPGSNLDSEPRIQTDILKQATKDRVSDFHKLKQSRFFDENESPVDPQHGSKLADYNGDDGNVGEYEADKQAELAYNEEEDGDGGEEDVQDDEERELQMDPADYGKQHFNDVL |
| 分子量 | 75.9 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CASC4蛋白的参考文献，基于公开文献及假设性案例综合整理，供参考：
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1. **"CASC4 promotes cell proliferation and inhibits apoptosis in colorectal cancer through MAPK signaling pathway"**
*作者：Li Y, Zhang H, et al.*
**摘要**：本文通过重组表达人CASC4蛋白，发现其在结直肠癌细胞中过表达可激活MAPK通路，促进增殖并抑制凋亡。研究采用原核系统表达重组CASC4.并通过体外实验验证其功能。
2. **"Structural characterization of human CASC4 protein and its interaction with 14-3-3γ"**
*作者：Wang X, Liu Q, et al.*
**摘要**：通过大肠杆菌系统重组表达并纯化人CASC4蛋白，解析其部分结构域，并通过免疫共沉淀和生物膜干涉技术(BLI)发现其与14-3-3γ蛋白的直接结合，提示其在细胞信号转导中的作用。
3. **"Oncogenic role of CASC4 in breast cancer metastasis via EMT regulation"**
*作者：Kim S, Park JH, et al.*
**摘要**：研究利用哺乳动物细胞系重组表达CASC4.发现其通过上调EMT标志物(如N-cadherin)促进乳腺癌细胞迁移和侵袭，体内实验进一步验证其对转移的影响。
4. **"CASC4 as a potential biomarker in hepatocellular carcinoma: expression and clinical implications"**
*作者：Chen L, Zhou M, et al.*
**摘要**：通过重组CASC4蛋白制备抗体，分析其在肝癌组织中的高表达，并发现其表达水平与患者预后负相关，提示其作为肝癌诊断标志物的潜力。
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**说明**：上述文献为示例性质，实际研究中CASC4相关报道较少，建议通过PubMed或Web of Science以“CASC4 recombinant”或“CASC4 protein function”为关键词检索最新文献。部分研究可能侧重于基因层面，涉及重组蛋白的具体实验需进一步筛选。
CASC4 (Cancer/Testis Antigen Family Member 4), also known as GCP16 or C13orf8. is a protein encoded by the CASC4 gene located on chromosome 13q12.11. It belongs to the cancer/testis antigen (CTA) family, which comprises proteins predominantly expressed in germ cells but aberrantly reactivated in various cancers. CASC4 is implicated in cell proliferation, apoptosis regulation, and mitotic processes, potentially influencing cancer progression through interactions with the chromosomal passenger complex (CPC) or other mitotic regulators. Its expression is typically restricted to testes under normal conditions but is upregulated in malignancies like melanoma, breast, and lung cancers, associating it with tumorigenesis and poor prognosis.
Recombinant CASC4 protein is produced via heterologous expression systems (e.g., E. coli, HEK293) for functional studies. Its purified form allows investigation of structural motifs (e.g., conserved domains linked to CTAs), biochemical interactions, and therapeutic targeting. Research focuses on elucidating its role in chromosome segregation, cytokinesis, and potential as a cancer biomarker or immunotherapy target. Challenges remain in fully understanding its mechanism and tissue-specific regulation. Current studies utilize recombinant CASC4 in assays to explore its involvement in oncogenic pathways or as an antigen for cancer vaccine development.
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