| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CASC3 |
| Uniprot No | O15234 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-703aa |
| 氨基酸序列 | MADRRRQRAS QDTEDEESGA SGSDSGGSPL RGGGSCSGSA GGGGSGSLPS QRGGRTGALH LRRVESGGAK SAEESECESE DGIEGDAVLS DYESAEDSEG EEGEYSEEEN SKVELKSEAN DAVNSSTKEE KGEEKPDTKS TVTGERQSGD GQESTEPVEN KVGKKGPKHL DDDEDRKNPA YIPRKGLFFE HDLRGQTQEE EVRPKGRQRK LWKDEGRWEH DKFREDEQAP KSRQELIALY GYDIRSAHNP DDIKPRRIRK PRYGSPPQRD PNWNGERLNK SHRHQGLGGT LPPRTFINRN AAGTGRMSAP RNYSRSGGFK EGRAGFRPVE AGGQHGGRSG ETVKHEISYR SRRLEQTSVR DPSPEADAPV LGSPEKEEAA SEPPAAAPDA APPPPDRPIE KKSYSRARRT RTKVGDAVKL AEEVPPPPEG LIPAPPVPET TPTPPTKTGT WEAPVDSSTS GLEQDVAQLN IAEQNWSPGQ PSFLQPRELR GMPNHIHMGA GPPPQFNRME EMGVQGGRAK RYSSQRQRPV PEPPAPPVHI SIMEGHYYDP LQFQGPIYTH GDSPAPLPPQ GMLVQPGMNL PHPGLHPHQT PAPLPNPGLY PPPVSMSPGQ PPPQQLLAPT YFSAPGVMNF GNPSYPYAPG ALPPPPPPHL YPNTQAPSQV YGGVTYYNPA QQQVQPKPSP PRRTPQPVTI KPPPPEVVSR GSS |
| 分子量 | 76.2 KDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **Title**: "CASC3 promotes colorectal cancer progression through regulating mRNA alternative splicing"
**Author**: Smith J, et al. (2018)
**Summary**: 研究揭示CASC3通过调控前体mRNA的可变剪接促进结直肠癌细胞增殖和迁移，机制涉及与剪接因子的相互作用。
2. **Title**: "Structural insights into human CASC3 protein in the exon junction complex"
**Author**: Johnson L, et al. (2020)
**Summary**: 通过晶体结构解析，阐明了CASC3蛋白在RNA外显子连接复合体(EJC)中的构象及其RNA结合域的关键功能。
3. **Title**: "CASC3 deficiency disrupts neurodevelopment via aberrant RNA processing in Drosophila"
**Author**: Zhang Y, et al. (2021)
**Summary**: 利用果蝇模型发现CASC3缺失导致神经元发育异常，表明其在神经系统RNA质量控制中的重要作用。
4. **Title**: "CASC3 mediates chemoresistance in esophageal squamous cell carcinoma by stabilizing anti-apoptotic transcripts"
**Author**: Wang R, et al. (2022)
**Summary**: 发现CASC3通过结合并稳定抗凋亡mRNA(如BCL2)，增强食管鳞癌对化疗药物的抵抗，提出其作为治疗靶点潜力。
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注：以上文献为示例，实际引用时需核对真实文献信息及DOI。
CASC3 (Cancer Susceptibility Candidate 3), also known as MLN51 or BTZ, is a multifunctional protein involved in mRNA metabolism and cellular stress responses. It is a core component of the Exon Junction Complex (EJC), which plays a critical role in post-transcriptional gene regulation, including mRNA splicing, surveillance, localization, and translation. CASC3 binds directly to spliced mRNAs during EJC assembly, facilitating interactions with downstream factors like UPF2 to mediate nonsense-mediated mRNA decay (NMD), a quality control mechanism that degrades transcripts with premature stop codons. Beyond NMD, CASC3 participates in DNA damage response pathways, cell cycle regulation, and apoptotic signaling under stress conditions. Dysregulation of CASC3 has been linked to cancers, neurological disorders, and developmental defects. Recombinant human CASC3 protein is typically produced in eukaryotic expression systems (e.g., HEK293 cells) to ensure proper post-translational modifications and functional activity. It serves as a vital tool for studying EJC dynamics, RNA-protein interactions, and mechanisms underlying diseases associated with mRNA processing errors. Research leveraging recombinant CASC3 also explores its potential as a biomarker or therapeutic target in oncology and genetic disorders.
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