| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CART1 |
| Uniprot No | Q9BUZ4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-470aa |
| 氨基酸序列 | MPGFDYKFLE KPKRRLLCPL CGKPMREPVQ VSTCGHRFCD TCLQEFLSEG VFKCPEDQLP LDYAKIYPDP ELEVQVLGLP IRCIHSEEGC RWSGPLRHLQ GHLNTCSFNV IPCPNRCPMK LSRRDLPAHL QHDCPKRRLK CEFCGCDFSG EAYESHEGMC PQESVYCENK CGARMMRRLL AQHATSECPK RTQPCTYCTK EFVFDTIQSH QYQCPRLPVA CPNQCGVGTV AREDLPGHLK DSCNTALVLC PFKDSGCKHR CPKLAMARHV EESVKPHLAM MCALVSRQRQ ELQELRRELE ELSVGSDGVL IWKIGSYGRR LQEAKAKPNL ECFSPAFYTH KYGYKLQVSA FLNGNGSGEG THLSLYIRVL PGAFDNLLEW PFARRVTFSL LDQSDPGLAK PQHVTETFHP DPNWKNFQKP GTWRGSLDES SLGFGYPKFI SHQDIRKRNY VRDDAVFIRA AVELPRKILS |
| 分子量 | 53.5 KDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为模拟示例的参考文献格式建议(实际文献需通过学术数据库检索验证)：
1. **文献名称**: "Cloning and functional analysis of recombinant human CART1 in osteogenic differentiation"
**作者**: Smith AB, et al.
**摘要**: 研究报道了重组人CART1蛋白的克隆表达及其在成骨细胞分化中的调控作用，发现其通过激活BMP信号通路促进骨形成。
2. **文献名称**: "Structural characterization of CART1 protein and its role in cartilage development"
**作者**: Chen X, Wang Y.
**摘要**: 通过X射线晶体学解析了CART1蛋白的三维结构，并验证其在软骨细胞分化和关节发育中的关键功能。
3. **文献名称**: "Recombinant CART1 as a potential therapeutic target in osteoarthritis"
**作者**: Lee S, et al.
**摘要**: 探讨重组CART1蛋白在小鼠骨关节炎模型中的治疗效果，显示其可抑制炎症因子释放并促进软骨修复。
4. **文献名称**: "CART1 regulates adipocyte differentiation via epigenetic modulation"
**作者**: Tanaka K, et al.
**摘要**: 揭示CART1通过表观遗传机制调控脂肪细胞分化的新功能，重组蛋白实验证实其与DNA甲基化酶的相互作用。
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**注意事项**：
1. 以上文献为示例格式，实际引用需检索PubMed、Web of Science等平台，使用关键词“recombinant CART1 protein”或“CART1 function”等。
2. 确认CART1蛋白的全称(如Cartilage-associated transcription factor 1)以精准筛选文献。
3. 建议优先选择近五年内发表的文献以反映研究前沿。
Cartilage-associated protein (CART1), also known as CART1 or CRTAC1. is a secreted extracellular matrix protein primarily expressed in chondrocytes and osteoblasts. It was initially identified as a cartilage-specific molecule involved in skeletal development and maintenance. Structurally, human CART1 contains a signal peptide, a highly conserved N-terminal domain with homology to the immunoglobulin-like fold, and a C-terminal domain rich in leucine-rich repeats (LRR) that mediates protein-protein interactions. Studies suggest its role in regulating chondrocyte differentiation, proliferation, and matrix mineralization, potentially through interactions with growth factors like BMP-2 or TGF-β.
CART1 expression is tightly linked to bone formation processes, including endochondral ossification, and its dysregulation has been implicated in skeletal disorders such as osteoarthritis and osteoporosis. Recombinant human CART1 protein, typically produced in mammalian expression systems (e.g., HEK293 cells), retains post-translational modifications critical for its biological activity. It serves as a valuable tool for studying cartilage-bone interface biology, mechanotransduction, and regenerative therapies. Recent research also explores its potential involvement in cancer progression, particularly in tumors with osteoid matrix deposition. The protein’s dual role in promoting mineralization and modulating cellular responses positions it as a promising target for skeletal tissue engineering and disease intervention.
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