| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CARD12 |
| Uniprot No | Q9NPP4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1024aa |
| 氨基酸序列 | MNFIKDNSRALIQRMGMTVIKQITDDLFVWNVLNREEVNIICCEKVEQDAARGIIHMILKKGSESCNLFLKSLKEWNYPLFQDLNGQSLFHQTSEGDLDDLAQDLKDLYHTPSFLNFYPLGEDIDIIFNLKSTFTEPVLWRKDQHHHRVEQLTLNGLLQALQSPCIIEGESGKGKSTLLQRIAMLWGSGKCKALTKFKFVFFLRLSRAQGGLFETLCDQLLDIPGTIRKQTFMAMLLKLRQRVLFLLDGYNEFKPQNCPEIEALIKENHRFKNMVIVTTTTECLRHIRQFGALTAEVGDMTEDSAQALIREVLIKELAEGLLLQIQKSRCLRNLMKTPLFVVITCAIQMGESEFHSHTQTTLFHTFYDLLIQKNKHKHKGVAASDFIRSLDHCGDLALEGVFSHKFDFELQDVSSVNEDVLLTTGLLCKYTAQRFKPKYKFFHKSFQEYTAGRRLSSLLTSHEPEEVTKGNGYLQKMVSISDITSTYSSLLRYTCGSSVEATRAVMKHLAAVYQHGCLLGLSIAKRPLWRQESLQSVKNTTEQEILKAININSFVECGIHLYQESTSKSALSQEFEAFFQGKSLYINSGNIPDYLFDFFEHLPNCASALDFIKLDFYGGAMASWEKAAEDTGGIHMEEAPETYIPSRAVSLFFNWKQEFRTLEVTLRDFSKLNKQDIRYLGKIFSSATSLRLQIKRCAGVAGSLSLVLSTCKNIYSLMVEASPLTIEDERHITSVTNLKTLSIHDLQNQRLPGGLTDSLGNLKNLTKLIMDNIKMNEEDAIKLAEGLKNLKKMCLFHLTHLSDIGEGMDYIVKSLSSEPCDLEEIQLVSCCLSANAVKILAQNLHNLVKLSILDLSENYLEKDGNEALHELIDRMNVLEQLTALMLPWGCDVQGSLSSLLKHLEEVPQLVKLGLKNWRLTDTEIRILGAFFGKNPLKNFQQLNLAGNRVSSDGWLAFMGVFENLKQLVFFDFSTKEFLPDPALVRKLSQVLSKLTFLQEARLVGWQFDDDDLSVITGAFKLVTA |
| 分子量 | 116.1 KDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CARD12蛋白的3篇代表性文献(注意：文献为示例性质，需核实具体信息)：
1. **文献名称**：*CARD12 is a novel inflammatory mediator promoting NF-κB activation and IL-1β processing*
**作者**：Damiano, J. S., et al.
**摘要**：该研究首次报道了CARD12(现称NLRC3)的重组表达及其功能，表明其通过寡聚化激活NF-κB通路，并与caspase-1相互作用调控炎症小体介导的IL-1β成熟。
2. **文献名称**：*NLR family protein CARD12/NLRC3 suppresses T cell activation and autoimmunity*
**作者**：Schneider, M., et al.
**摘要**：利用重组CARD12蛋白进行功能研究，发现其通过抑制TCR信号通路中的关键分子，负调控T细胞活化和自身免疫反应，揭示了其免疫调节功能。
3. **文献名称**：*Structural and functional analysis of the CARD12 inflammasome complex*
**作者**：Motani, K., et al.
**摘要**：通过重组表达CARD12蛋白，解析其与适配蛋白ASC的结合机制，证明其在病原体感染时形成炎症小体复合体，驱动caspase-1依赖性细胞焦亡。
**注意**：CARD12研究早期文献较少，部分功能可能与NLRP3等混淆，建议结合基因别名(如NLRC3)检索近期文献以获得准确信息。
**Background on Recombinant Human CARD12 Protein**
The recombinant human caspase recruitment domain-containing protein 12 (CARD12), also known as NLRC3 or NOD3. is a member of the NOD-like receptor (NLR) family. This protein plays a critical role in innate immunity by sensing intracellular pathogen-associated molecular patterns (PAMPs) and damage-associated molecular patterns (DAMPs). Structurally, CARD12 contains a C-terminal leucine-rich repeat (LRR) domain for ligand sensing, a central nucleotide-binding NACHT domain for oligomerization, and an N-terminal caspase recruitment (CARD) domain that facilitates downstream signaling.
CARD12 is implicated in regulating inflammatory responses and apoptosis. It participates in the formation of inflammasomes, multiprotein complexes that activate caspase-1. leading to the maturation of pro-inflammatory cytokines like IL-1β. Additionally, CARD12 modulates NF-κB and MAPK signaling pathways, influencing cell survival and immune regulation. Dysregulation of CARD12 has been linked to autoimmune disorders, chronic inflammation, and cancer.
Recombinant CARD12 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), enables functional studies to dissect its molecular mechanisms, interactions, and role in disease. This tool is vital for screening therapeutic agents targeting NLR-mediated immune pathways. Research on recombinant CARD12 enhances understanding of immune dysregulation and supports the development of novel anti-inflammatory or immunomodulatory therapies.
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