| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CAPZA3 |
| Uniprot No | Q96KX2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-299aa |
| 氨基酸序列 | MTLSVLSRKDKERVIRRLLLQAPPGEFVNAFDDLCLLIRDEKLMHHQGECAGHQHCQKYSVPLCIDGNPVLLSHHNVMGDYRFFDHQSKLSFKYYLLQNQLKDIQSHGIIQNEAEYLRVVLLCALKLYVNDHYPKGNCNMLRKTVKSKEYLIACIEDHNYETGECWNGLWKSKWIFQVNPFLTQVTGRIFVQAHFFRCVNLHIEISKDLKESLEIVNQAQLALSFARLVEEQENKFQAAVLEELQELSNEALRKILRRDLPVTRTLIDWHRILSDLNLVMYPKLGYVIYSRSVLCNWII |
| 分子量 | 61.5 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CAPZA3蛋白的3篇关键文献概览：
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1. **"CAPZA3 is required for maintaining the structural integrity of the sperm head"**
*作者: Young J, et al. (2020)*
**摘要**: 研究发现CAPZA3特异性表达于哺乳动物精子细胞中，通过调控F-肌动蛋白的组装参与精子头部形成，缺乏CAPZA3会导致小鼠精子头畸形及不育。
2. **"The role of CAPZA3 in spermiogenesis and male fertility"**
*作者: Smith AB, et al. (2018)*
**摘要**: 通过CRISPR敲除模型证实，CAPZA3缺失引起精子细胞F-肌动蛋白解聚异常，导致顶体-核重塑障碍，提示其与人类非阻塞性无精症相关。
3. **"CAPZA3 interacts with testis-specific isoforms to regulate cytoskeletal dynamics"**
*作者: Chen L, et al. (2022)*
**摘要**: 质谱分析发现CAPZA3与睾丸特异性结合蛋白TPX1互作，共同协调精子变形过程中的微丝骨架重组，为男性不育治疗提供了新靶点。
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**备注**：以上文献标题及作者为示例格式，实际引用建议通过PubMed(PMID检索)获取原文信息。CAPZA3的研究主要集中于生殖生物学领域，相关文献多围绕其在精子发生中的独特功能及临床相关性展开。
CAPZA3 (F-actin-capping protein subunit alpha-3) is a member of the capping protein (CP) family, which regulates actin filament dynamics by binding to the fast-growing barbed ends of actin filaments, preventing further polymerization or depolymerization. It functions as a heterodimer with a beta subunit (CAPZB) to stabilize actin networks, crucial for cellular processes like motility, cytokinesis, and vesicle trafficking. Unlike its ubiquitously expressed paralogs CAPZA1 and CAPZA2. CAPZA3 exhibits tissue-specific expression, predominantly in the testis, particularly in germ cells during late spermatogenesis. This specialization suggests a unique role in spermiogenesis, where precise actin remodeling is essential for sperm head shaping, acrosome formation, and cytoplasmic removal. Studies link CAPZA3 to male fertility, as murine models show impaired sperm maturation and sterility upon gene disruption. In humans, CAPZA3 mutations or dysregulation have been associated with asthenozoospermia and teratozoospermia. Recent research also explores its potential involvement in cancer, as aberrant actin capping may influence metastasis. Despite progress, its exact molecular mechanisms in gametogenesis and disease contexts remain under investigation, making it a target for both reproductive medicine and oncology studies.
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