| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CALD1 |
| Uniprot No | Q05682 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-793aa |
| 氨基酸序列 | MDDFERRREL RRQKREEMRL EAERIAYQRN DDDEEEAARE RRRRARQERL RQKQEEESLG QVTDQVEVNA QNSVPDEEAK TTTTNTQVEG DDEAAFLERL ARREERRQKR LQEALERQKE FDPTITDASL SLPSRRMQND TAENETTEKE EKSESRQERY EIEETETVTK SYQKNDWRDA EENKKEDKEK EEEEEEKPKR GSIGENQVEV MVEEKTTESQ EETVVMSLKN GQISSEEPKQ EEEREQGSDE ISHHEKMEEE DKERAEAERA RLEAEERERI KAEQDKKIAD ERARIEAEEK AAAQERERRE AEERERMREE EKRAAEERQR IKEEEKRAAE ERQRIKEEEK RAAEERQRIK EEEKRAAEER QRARAEEEEK AKVEEQKRNK QLEEKKHAMQ ETKIKGEKVE QKIEGKWVNE KKAQEDKLQT AVLKKQGEEK GTKVQAKREK LQEDKPTFKK EEIKDEKIKK DKEPKEEVKS FMDRKKGFTE VKSQNGEFMT HKLKHTENTF SRPGGRASVD TKEAEGAPQV EAGKRLEELR RRRGETESEE FEKLKQKQQE AALELEELKK KREERRKVLE EEEQRRKQEE ADRKLREEEE KRRLKEEIER RRAEAAEKRQ KMPEDGLSDD KKPFKCFTPK GSSLKIEERA EFLNKSVQKS SGVKSTHQAA IVSKIDSRLE QYTSAIEGTK SAKPTKPAAS DLPVPAEGVR NIKSMWEKGN VFSSPTAAGT PNKETAGLKV GVSSRINEWL TKTPDGNKSP APKPSDLRPG DVSSKRNLWE KQSVDKVTSP TKV |
| 分子量 | 93.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇关于重组人钙桥蛋白(CALD1)的示例文献摘要(文献信息为示例，非真实存在)：
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1. **文献名称**: "Recombinant human caldesmon modulates smooth muscle contraction in vitro"
**作者**: Humphries, M.J. et al.
**摘要**: 研究利用重组CALD1蛋白分析其对平滑肌收缩的调控作用，发现其通过抑制肌动蛋白-肌球蛋白相互作用降低收缩力，揭示其在细胞骨架动态中的关键角色。
2. **文献名称**: "Caldesmon phosphorylation and interaction with calcium/calmodulin"
**作者**: Hayashi, K. & Yano, H.
**摘要**: 通过重组表达CALD1片段，揭示了其磷酸化依赖性结合钙调蛋白的分子机制，为理解细胞迁移中的信号传导提供了结构基础。
3. **文献名称**: "CALD1 promotes tumor invasion via actin cytoskeleton remodeling"
**作者**: Sharma, R. et al.
**摘要**: 在乳腺癌模型中，重组CALD1蛋白通过调控肌动蛋白纤维重组增强癌细胞侵袭能力，提示其作为潜在治疗靶点。
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*注：上述文献为生成示例，具体研究请查阅真实数据库(如PubMed)或补充完整基因/蛋白名称以获取准确信息。*
Caldesmon (CALD1) is a key cytoskeletal protein encoded by the CALD1 gene, primarily expressed in smooth muscle and non-muscle cells. It plays a critical role in regulating actin filament dynamics by binding to actin, tropomyosin, and myosin, thereby modulating cell contraction, migration, and proliferation. CALD1 exists as two major isoforms generated by alternative splicing: high-molecular-weight caldesmon (h-CALD1), predominantly found in smooth muscle, and low-molecular-weight caldesmon (l-CALD1), expressed in non-muscle cells. Both isoforms act as molecular scaffolds, integrating cytoskeletal organization with signaling pathways. In smooth muscle, CALD1 inhibits actomyosin ATPase activity under low calcium conditions, maintaining relaxation, while calcium-dependent phosphorylation by kinases (e.g., MAPK, CDK1) reverses this inhibition to enable contraction. Dysregulation of CALD1 is linked to pathological processes, including cancer progression, where its overexpression promotes tumor invasion, metastasis, and epithelial-mesenchymal transition (EMT) by enhancing cell motility and survival. Recombinant human CALD1 protein, typically produced in bacterial or mammalian expression systems, retains functional domains essential for binding partners and is widely used in vitro to study cytoskeletal mechanics, calcium signaling, and drug screening. Its role in vascular tone, wound healing, and tumorigenesis continues to make it a focus of biomedical research.
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