| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CALCRL |
| Uniprot No | Q16602 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 23-132aa |
| 氨基酸序列 | ELEESPEDSIQLGVTRNKIMTAQYECYQKIMQDPIQQAEGVYCNRTWDGWLCWNDVAAGTESMQLCPDYFQDFDPSEKVTKICDQDGNWFRHPASNRTWTNYTQCNVNTH |
| 分子量 | 37.84 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人降钙素基因相关肽1受体(CALCRL)的3篇参考文献摘要：
1. **文献名称**: *Structural basis of CGRP receptor signaling and regulation*
**作者**: Liang YL, et al.
**摘要**: 该研究通过冷冻电镜解析了CALCRL与RAMP1蛋白及CGRP配体的复合物结构，揭示了受体激活的分子机制，为靶向CGRP受体的药物设计(如偏头痛治疗)提供了结构基础。
2. **文献名称**: *CALCRL is a critical regulator of angiogenesis in pulmonary hypertension*
**作者**: Smith CJ, Koyama T.
**摘要**: 研究发现CALCRL通过与肾上腺髓质素(AM)结合，在肺动脉高压中调控血管生成和内皮细胞功能，为靶向该通路治疗心血管疾病提供了新思路。
3. **文献名称**: *The role of CALCRL in neuropathic pain and migraine pathophysiology*
**作者**: Russo AF, et al.
**摘要**: 综述了CALCRL在神经痛和偏头痛中的核心作用，强调其与CGRP信号通路的关联，并总结当前靶向该受体药物的临床应用进展及挑战。
以上文献涵盖结构解析、疾病机制和治疗应用，均为该领域关键研究。
The calcitonin receptor-like receptor (CALCRL) is a class B G protein-coupled receptor (GPCR) that plays a critical role in vascular, neurological, and metabolic regulation. Initially identified through homology with the calcitonin receptor, CALCRL does not bind calcitonin but forms functional complexes with receptor activity-modifying proteins (RAMPs). When paired with RAMP1. it constitutes the calcitonin gene-related peptide (CGRP) receptor, which mediates vasodilation, nociception, and inflammatory responses. In combination with RAMP2 or RAMP3. CALCRL forms the adrenomedullin receptor, implicated in cardiovascular homeostasis, angiogenesis, and fluid balance.
CALCRL's signaling involves coupling with Gαs and Gαq proteins, activating pathways like cAMP/PKA and PLC/IP3. Dysregulation of CALCRL-associated pathways is linked to migraines, hypertension, heart failure, and cancer. The receptor's role in migraine pathophysiology has driven the development of CGRP-targeted therapies, including monoclonal antibodies and small-molecule antagonists. Structural studies of CALCRL-RAMP complexes have provided insights into ligand specificity and allosteric modulation, guiding drug design. Despite progress, challenges remain in understanding tissue-specific signaling and optimizing therapeutic strategies to minimize side effects. CALCRL remains a focal point for research in GPCR biology and precision medicine.
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