关键信息
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基因名
SMNDC1
- 应用
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别名
SMNDC1;SMNR;SPF30;Survival of motor neuron-related-splicing factor 30
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O75940
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表达区间
1-238aa
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氨基酸序列
MGSSHHHHHHSSGLVPRGSHMSEDLAKQLASYKAQLQQVEAALSGNGENE DLLKLKKDLQEVIELTKDLLSTQPSETLASSDSFASTQPTHSWKVGDKCM AVWSEDGQCYEAEIEEIDEENGTAAITFAGYGNAEVTPLLNLKPVEEGRK AKEDSGNKPMSKKEMIAQQREYKKKKALKKAQRIKELEQEREDQKVKWQQ FNNRAYSKNKKGQVKRSIFASPESVTGKVGVGTCGIADKPMTQYQDTSKY NVRHLMPQ
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分子量
29 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
SMNDC1, or Survival of Motor Neuron Domain Containing 1, is a protein that plays a crucial role in the assembly of spliceosomal snRNPs (small nuclear ribonucleoproteins), which are essential for pre-mRNA splicing, a fundamental process in gene expression regulation. Mutations in the SMN1 gene lead to spinal muscular atrophy (SMA), a severe neurodegenerative disorder characterized by the loss of motor neurons and muscle weakness. The discovery of SMNDC1 has emerged as an important area of research, particularly due to its potential therapeutic implications in SMA and other neurological conditions. Understanding the structure, function, and interactions of SMNDC1 can provide insights into the molecular mechanisms underlying splicing and neurodegeneration. Furthermore, the production of recombinant SMNDC1 protein allows for biochemical studies, high-throughput screening for small molecules, and identification of potential drug targets. The investigation of SMNDC1 has the potential to not only advance our comprehension of RNA biology but also pave the way for developing novel interventions in diseases linked to splicing defects, presenting a promising frontier in molecular medicine and therapeutics.
