| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CACNB3 |
| Uniprot No | P54284 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-484aa |
| 氨基酸序列 | MYDDSYVPGF EDSEAGSADS YTSRPSLDSD VSLEEDRESA RREVESQAQQ QLERAKHKPV AFAVRTNVSY CGVLDEECPV QGSGVNFEAK DFLHIKEKYS NDWWIGRLVK EGGDIAFIPS PQRLESIRLK QEQKARRSGN PSSLSDIGNR RSPPPSLAKQ KQKQAEHVPP YDVVPSMRPV VLVGPSLKGY EVTDMMQKAL FDFLKHRFDG RISITRVTAD LSLAKRSVLN NPGKRTIIER SSARSSIAEV QSEIERIFEL AKSLQLVVLD ADTINHPAQL AKTSLAPIIV FVKVSSPKVL QRLIRSRGKS QMKHLTVQMM AYDKLVQCPP ESFDVILDEN QLEDACEHLA EYLEVYWRAT HHPAPGPGLL GPPSAIPGLQ NQQLLGERGE EHSPLERDSL MPSDEASESS RQAWTGSSQR SSRHLEEDYA DAYQDLYQPH RQHTSGLPSA NGHDPQDRLL AQDSEHNHSD RNWQRNRPWP KDSY |
| 分子量 | 80.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CACNB3蛋白的模拟参考文献示例(仅供格式参考，非真实文献)：
1. **文献名称**：*CACNB3 modulates voltage-gated calcium channel function in cardiomyocytes*
**作者**：Li X, et al.
**摘要**：研究通过表达重组人CACNB3蛋白，发现其在心肌细胞中调控L型钙通道的门控特性，影响动作电位持续时间，可能与心律失常发生相关。
2. **文献名称**：*Structural insights into the human CACNB3 subunit interaction with Cav1.2*
**作者**：Zhang Y, et al.
**摘要**：利用X射线晶体学解析了重组CACNB3蛋白与Cav1.2钙通道α亚基的结合界面，揭示了β3亚基通过保守结构域稳定通道开放的分子机制。
3. **文献名称**：*A CACNB3 mutation linked to Brugada syndrome disrupts calcium channel trafficking*
**作者**：Giudicessi JR, et al.
**摘要**：临床研究发现CACNB3基因突变导致重组蛋白的胞内运输缺陷，减少细胞膜钙通道密度，可能导致Brugada综合征的电生理异常。
4. **文献名称**：*CACNB3 knockout mice exhibit altered synaptic transmission*
**作者**：Wang H, et al.
**摘要**：通过构建CACNB3基因敲除小鼠模型，发现β3亚基缺失导致海马神经元突触钙内流减少，提示其在神经信号传导中的调控作用。
**提示**：以上为模拟内容，实际文献需通过PubMed或Web of Science检索关键词(如“CACNB3 recombinant protein”“CACNB3 calcium channel”)获取。
Recombinant human CACNB3 protein, derived from the CACNB3 gene, encodes the β3 subunit of voltage-gated calcium (Ca²⁺) channels. These channels are critical for regulating calcium ion influx in excitable cells, influencing processes like muscle contraction, neurotransmitter release, and gene expression. The β3 subunit, part of the β-subunit family (β1-β4), modulates the trafficking and biophysical properties of the pore-forming α1 subunit of L-type calcium channels (CaV1.2/CaV1.3). Structurally, CACNB3 contains conserved Src homology 3 (SH3) and guanylate kinase (GK) domains, enabling interactions with the α1 subunit to stabilize channel complexes and fine-tune voltage-dependent activation/inactivation kinetics.
Primarily expressed in skeletal muscle, heart, and brain, CACNB3 variants or dysregulation are linked to cardiac arrhythmias (e.g., Brugada syndrome) and neurological disorders. Recombinant CACNB3 is utilized in research to explore its regulatory roles in calcium signaling and disease mechanisms. Studies suggest its involvement in cancer progression due to altered expression in certain tumors. Engineered via heterologous expression systems (e.g., *E. coli* or mammalian cells), recombinant CACNB3 retains functional properties, aiding structural analyses and drug screening for channelopathies. Its interaction with auxiliary α2δ subunits further highlights its importance in channel assembly and therapeutic targeting.
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