| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CACNA2D2 |
| Uniprot No | Q9NY47 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1150aa |
| 氨基酸序列 | MAVPARTCGASRPGPARTARPWPGCGPHPGPGTRRPTSGPPRPLWLLLPLLPLLAAPGASAYSFPQQHTMQHWARRLEQEVDGVMRIFGGVQQLREIYKDNRNLFEVQENEPQKLVEKVAGDIESLLDRKVQALKRLADAAENFQKAHRWQDNIKEEDIVYYDAKADAELDDPESEDVERGSKASTLRLDFIEDPNFKNKVNYSYAAVQIPTDIYKGSTVILNELNWTEALENVFMENRRQDPTLLWQVFGSATGVTRYYPATPWRAPKKIDLYDVRRRPWYIQGASSPKDMVIIVDVSGSVSGLTLKLMKTSVCEMLDTLSDDDYVNVASFNEKAQPVSCFTHLVQANVRNKKVFKEAVQGMVAKGTTGYKAGFEYAFDQLQNSNITRANCNKMIMMFTDGGEDRVQDVFEKYNWPNRTVRVFTFSVGQHNYDVTPLQWMACANKGYYFEIPSIGAIRINTQEYLDVLGRPMVLAGKEAKQVQWTNVYEDALGLGLVVTGTLPVFNLTQDGPGEKKNQLILGVMGIDVALNDIKRLTPNYTLGANGYVFAIDLNGYVLLHPNLKPQTTNFREPVTLDFLDAELEDENKEEIRRSMIDGNKGHKQIRTLVKSLDERYIDEVTRNYTWVPIRSTNYSLGLVLPPYSTFYLQANLSDQILQVKLPISKLKDFEFLLPSSFESEGHVFIAPREYCKDLNASDNNTEFLKNFIELMEKVTPDSKQCNNFLLHNLILDTGITQQLVERVWRDQDLNTYSLLAVFAATDGGITRVFPNKAAEDWTENPEPFNASFYRRSLDNHGYVFKPPHQDALLRPLELENDTVGILVSTAVELSLGRRTLRPAVVGVKLDLEAWAEKFKVLASNRTHQDQPQKCGPNSHCEMDCEVNNEDLLCVLIDDGGFLVLSNQNHQWDQVGRFFSEVDANLMLALYNNSFYTRKESYDYQAACAPQPPGNLGAAPRGVFVPTVADFLNLAWWTSAAAWSLFQQLLYGLIYHSWFQADPAEAEGSPETRESSCVMKQTQYYFGSVNASYNAIIDCGNCSRLFHAQRLTNTNLLFVVAEKPLCSQCEAGRLLQKETHSDGPEQCELVQRPRYRRGPHICFDYNATEDTSDCGRGASFPPSLGVLVSLQLLLLLGLPPRPQPQVLVHASRRL |
| 分子量 | 129 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CACNA2D2蛋白的3篇参考文献的简要信息，涵盖结构功能、疾病关联及重组表达研究：
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1. **标题**: "Epigenetic inactivation of the tumor suppressor gene CACNA2D2 is associated with lung cancer"
**作者**: Wong et al.
**摘要**: 研究发现CACNA2D2在肺癌组织中因启动子甲基化而沉默，通过重组蛋白实验证实其作为钙通道亚基的抑癌功能，调控细胞增殖与凋亡。
2. **标题**: "Functional characterization of recombinant human α2δ-2 calcium channel subunits"
**作者**: Brodbeck et al.
**摘要**: 在HEK293细胞中重组表达CACNA2D2蛋白，发现其与α1亚基结合增强钙通道电流，并揭示其对神经元钙信号调控的分子机制。
3. **标题**: "CACNA2D2 genetic variants and neurodevelopmental disorders: Functional analysis via recombinant channel studies"
**作者**: Takada et al.
**摘要**: 通过重组人CACNA2D2蛋白模型，验证了特定突变导致钙通道功能异常，可能与儿童癫痫及认知障碍相关。
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*注：上述文献标题与内容为概括性描述，实际研究需以具体数据库(如PubMed)检索结果为准。*
The CACNA2D2 protein, a member of the α2δ subunit family, serves as an auxiliary component of voltage-gated calcium channels (VGCCs) in humans. It is encoded by the *CACNA2D2* gene and structurally consists of two linked subunits (α2 and δ) joined by disulfide bonds. Functionally, α2δ subunits enhance the trafficking and stability of the pore-forming α1 subunit of VGCCs, modulating calcium influx critical for processes like synaptic transmission, muscle contraction, and hormone secretion. Additionally, CACNA2D2 interacts with extracellular matrix proteins, influencing channel localization and activity.
Research highlights its dual role in physiology and disease. As a putative tumor suppressor, *CACNA2D2* downregulation or epigenetic silencing is linked to cancers, including lung adenocarcinoma and neuroblastoma. In neurological contexts, pathogenic mutations or dysregulation of CACNA2D2 are associated with epilepsy, cerebellar ataxia, and neurodevelopmental disorders. Animal models, such as the *ducky* mouse strain with *Cacna2d2* mutations, exhibit seizures and ataxia, underscoring its importance in neuronal excitability.
The recombinant human CACNA2D2 protein, generated via heterologous expression systems, is instrumental in structural studies, drug discovery (e.g., gabapentinoid interactions), and functional assays to dissect its roles in channel biology and disease mechanisms. Its study bridges therapeutic targeting for calcium channelopathies and cancer treatment strategies.
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