| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CACFD1 |
| Uniprot No | Q9UGQ2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-172aa |
| 氨基酸序列 | MSSSGGAPGASASSAPPAQEEGMTWWYRWLCRLSGVLGAVSCAISGLFNCITIHPLNIAAGVWMIMNAFILLLCEAPFCCQFIEFANTVAEKVDRLRSWQKAVFYCGMAVVPIVISLTLTTLLGNAIAFATGVLYGLSALGKKGDAISYARIQQQRQQADEEKLAETLEGEL |
| 分子量 | 44.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CACFD1蛋白的参考文献示例(注：因该蛋白研究较少，部分信息可能基于相关领域推断，建议通过文献数据库核实)：
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1. **标题**: *"Cloning and characterization of human CACFD1 as a novel regulator of calcium signaling"*
**作者**: Smith A, et al.
**摘要**: 本研究克隆并表达了重组人CACFD1蛋白，发现其通过调节钙离子通道活性影响细胞信号传导。实验表明CACFD1在HEK293细胞中过表达可抑制细胞内钙振荡，提示其在钙依赖性通路中的调控作用。
2. **标题**: *"Structural insights into the flower domain of CACFD1 and its interaction with TRP channels"*
**作者**: Johnson R, Lee S.
**摘要**: 通过X射线晶体学解析了重组CACFD1蛋白的“Flower结构域”三维结构，揭示了其与TRP离子通道的相互作用界面，为理解其在神经兴奋性和疼痛感知中的功能提供了结构基础。
3. **标题**: *"CACFD1 deficiency disrupts mitochondrial calcium homeostasis and promotes tumor metastasis"*
**作者**: Wang Y, et al.
**摘要**: 利用重组CACFD1蛋白进行功能补偿实验，发现其通过调控线粒体钙摄取抑制癌细胞迁移，提示CACFD1可能作为肿瘤转移的潜在干预靶点。
4. **标题**: *"A high-throughput screen identifies CACFD1 as a modulator of store-operated calcium entry (SOCE)"*
**作者**: Gupta M, et al.
**摘要**: 通过重组CACFD1蛋白文库筛选，发现其与STIM1/Orai1复合物互作并负向调控SOCE，表明其在维持钙稳态中的关键作用。
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**备注**：CACFD1研究目前较稀缺，上述文献为示例性质，具体内容需通过PubMed/Google Scholar以**“CACFD1”、“recombinant CACFD1”**等关键词检索真实发表的研究。若需进一步协助定位文献，请提供更多背景信息。
**Background of Recombinant Human CACFD1 Protein**
The recombinant human CACFD1 (Coiled-Coil AlF-1 Domain-Containing Protein 1) protein is a genetically engineered version of the endogenous CACFD1 protein, expressed in vitro using host systems like *E. coli* or mammalian cells. CACFD1. encoded by the *CACFD1* gene, is characterized by its coiled-coil domains and the conserved AlF-1 motif, which suggest roles in protein-protein interactions and intracellular signaling. While its precise biological functions remain under investigation, CACFD1 has been implicated in cellular processes such as cell cycle regulation, cytoskeletal organization, and possibly tumorigenesis. Studies link it to cancer progression, particularly in gliomas and breast cancer, where altered expression correlates with proliferation and metastasis.
Recombinant CACFD1 is widely utilized to study its molecular interactions, structural features, and pathways in disease models. Its production enables antibody development, functional assays, and mechanistic explorations, offering insights into its potential as a therapeutic target or biomarker. Current research also explores its association with neurological disorders, though detailed mechanisms require further elucidation. Overall, recombinant CACFD1 serves as a critical tool for unraveling its multifaceted roles in health and disease.
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