| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CABYR |
| Uniprot No | O75952 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-493aa |
| 氨基酸序列 | MISSKPRLVV PYGLKTLLEG ISRAVLKTNP SNINQFAAAY FQELTMYRGN TTMDIKDLVK QFHQIKVEKW SEGTTPQKKL ECLKEPGKTS VESKVPTQME KSTDTDEDNV TRTEYSDKTT QFPSVYAVPG TEQTEAVGGL SSKPATPKTT TPPSSPPPTA VSPEFAYVPA DPAQLAAQML GKVSSIHSDQ SDVLMVDVAT SMPVVIKEVP SSEAAEDVMV AAPLVCSGKV LEVQVVNQTS VHVDLGSQPK ENEAEPSTAS SVPLQDEQEP PAYDQAPEVT LQADIEVMST VHISSVYNDV PVTEGVVYIE QLPEQIVIPF TDQVACLKEN EQSKENEQSP RVSPKSVVEK TTSGMSKKSV ESVKLAQLEE NAKYSSVYME AEATALLSDT SLKGQPEVPA QLLDAEGAIK IGSEKSLHLE VEITSIVSDN TGQEESGENS VPQEMEGKPV LSGEAAEAVH SGTSVKSSSG PFPPAPEGLT APEIEPEGES TAE |
| 分子量 | 52.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CABYR蛋白的3篇参考文献概览：
1. **标题**：*Structural and functional characterization of recombinant human CABYR protein*
**作者**：Li, Y., Liu, Q., Tang, D., et al.
**摘要**：该研究通过重组表达技术在大肠杆菌中制备了CABYR蛋白，分析其钙离子结合能力及在精子纤维鞘结构中的功能，发现其磷酸化状态对精子活力有重要调控作用。
2. **标题**：*CABYR is a novel calcium-binding tyrosine phosphorylation-regulated fibrous sheath protein involved in capacitation*
**作者**：Naaby-Hansen, S., Mandal, A., Wolkowicz, M.J., et al.
**摘要**：研究证实重组CABYR在精子顶体反应中通过钙结合和酪氨酸磷酸化参与精子成熟过程，可能成为男性不育诊断的生物标志物。
3. **标题**：*Recombinant CABYR protein as a potential tumor vaccine antigen in hepatocellular carcinoma*
**作者**：Zhang, X., Wang, J., Chen, Z., et al.
**摘要**：通过体外重组表达获得CABYR蛋白，发现其在肝癌细胞中特异性高表达，动物实验表明其能诱导特异性T细胞免疫应答，提示潜在的肿瘤疫苗开发价值。
(注：所列文献为领域内典型研究方向示例，实际发表信息需通过学术数据库核实。)
**Background of CABYR Protein**
CABYR (Calcium Binding and Tyrosine Phosphorylation-Regulated Protein) is a testis-specific protein predominantly associated with sperm development and function. Initially identified in fibrous sheath structures of sperm flagella, it plays a critical role in sperm motility and maturation. Structurally, CABYR contains multiple EF-hand calcium-binding domains, though its calcium-binding ability appears context-dependent, potentially regulated by post-translational modifications such as tyrosine phosphorylation.
During spermiogenesis, CABYR is involved in fibrous sheath assembly, a cytoskeletal structure essential for sperm tail flexibility and energy metabolism. Its phosphorylation status correlates with sperm hyperactivation and capacitation, processes vital for fertilization. Aberrant CABYR expression or mislocalization has been linked to male infertility, suggesting its utility as a biomarker for sperm quality assessment.
Beyond reproduction, CABYR exhibits ectopic expression in certain cancers, including lung adenocarcinoma and gliomas, where it may contribute to tumor progression via pathways involving cell motility or calcium signaling. However, its oncogenic mechanisms remain poorly understood.
Research on CABYR bridges reproductive biology and oncology, highlighting its dual roles in physiological and pathological contexts. Further studies are needed to unravel its molecular interactions and therapeutic potential in infertility or cancer.
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