| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CABP5 |
| Uniprot No | Q9NP86 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-173aa |
| 氨基酸序列 | MQFPMGPACIFLRKGIAEKQRERPLGQDEIEELREAFLEFDKDRDGFISCKDLGNLMRTMGYMPTEMELIELGQQIRMNLGGRVDFDDFVELMTPKLLAETAGMIGVQEMRDAFKEFDTNGDGEITLVELQQAMQRLLGERLTPREISEVVREADVNGDGTVDFEEFVKMMSR |
| 分子量 | 19.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CABP5蛋白的3条参考文献示例(注：文献为虚构，仅作示例参考)：
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1. **标题**：*"Recombinant human CABP5 expression and its calcium-dependent interaction with Cav1.4 channels"*
**作者**：Haeseleer F, et al.
**摘要**：本研究在大肠杆菌中成功表达了重组人CABP5蛋白，并通过pull-down实验证实其与视网膜感光细胞中的CaV1.4钙通道直接结合，揭示了CABP5在钙依赖性通道失活中的调控作用。
2. **标题**：*"Structural characterization of CABP5 using cryo-EM reveals novel calcium-binding motifs"*
**作者**：Peterson SB, et al.
**摘要**：通过冷冻电镜解析重组人CABP5的高分辨率结构，鉴定出其独特的EF-hand钙结合域，并探讨了该结构对其在神经元突触传递中调节钙稳态的功能意义。
3. **标题**：*"CABP5 autoantibodies as a biomarker for autoimmune retinopathy: Development of a recombinant protein-based diagnostic assay"*
**作者**：Zhang Y, et al.
**摘要**：利用重组人CABP5蛋白开发新型ELISA检测方法，证实其在自身免疫性视网膜病变患者血清中特异性抗体的检测能力，为临床诊断提供新工具。
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**注**：上述文献为示例，实际研究中请通过权威数据库(如PubMed、Web of Science)检索真实发表的文献。建议使用关键词“CABP5”“calcium-binding protein 5”“recombinant expression”进行拓展查询。
CABP5 (calcium-binding protein 5) is a member of the neuronal calcium-binding protein family, primarily expressed in retinal neurons, including photoreceptors and bipolar cells. It plays a critical role in regulating calcium-mediated signaling pathways, which are essential for visual signal transduction. Structurally, CABP5 contains conserved EF-hand domains that enable selective binding to Ca²⁺ ions, modulating intracellular calcium homeostasis and interactions with target proteins like guanylate cyclase.
Recombinant human CABP5 protein is engineered using expression systems (e.g., E. coli or mammalian cells) to produce purified, functional protein for research. Its study aids in elucidating mechanisms of calcium-dependent processes in retinal physiology and pathology. Dysregulation of CABP5 has been linked to retinal degenerative diseases, such as cone-rod dystrophy, and aberrant expression in certain cancers suggests broader roles in cellular proliferation and apoptosis.
Research on recombinant CABP5 also explores its potential as a diagnostic biomarker or therapeutic target. Recent studies investigate its interplay with photoreceptor-specific proteins and calcium signaling cascades, offering insights into vision-related disorders. Advances in protein engineering enhance its stability and usability in structural studies, facilitating drug discovery and mechanistic investigations. Further work aims to clarify its tissue-specific functions and therapeutic applications in calcium-associated diseases.
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