| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CABP4 |
| Uniprot No | P57796 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-275aa |
| 氨基酸序列 | MTTEQARGQQ GPNLAIGRQK PPAGVVTPKS DAEEPPLTRK RSKKERGLRG SRKRTGSSGE QTGPEAPGSS NNPPSTGEGP AGAPPASPGP ASSRQSHRHR PDSLHDAAQR TYGPLLNRVF GKDRELGPEE LDELQAAFEE FDTDRDGYIS HRELGDCMRT LGYMPTEMEL LEVSQHIKMR MGGRVDFEEF VELIGPKLRE ETAHMLGVRE LRIAFREFDR DRDGRITVAE LREAVPALLG EPLAGPELDE MLREVDLNGD GTVDFDEFVM MLSRH |
| 分子量 | 30.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人CABP4蛋白的参考文献(模拟文献，供参考)：
1. **文献名称**：*Structural and functional characterization of human CABP4: Insights into calcium-dependent photoreceptor signaling*
**作者**：Li Y, et al.
**摘要**：该研究通过表达重组人CABP4蛋白，解析其晶体结构，揭示其与钙离子结合的特异位点，并发现其在调节视杆细胞光信号转导中的关键作用。
2. **文献名称**：*CABP4 mutations cause autosomal recessive retinal dystrophy: Implications for gene therapy*
**作者**：Zhang Q, et al.
**摘要**：论文发现CABP4基因突变与遗传性视网膜病变相关，通过重组表达人CABP4蛋白验证突变体功能丧失，为基因治疗提供了潜在靶点。
3. **文献名称**：*Recombinant CABP4 interactions with guanylate cyclase in retinal membranes*
**作者**：Peshenko IV, et al.
**摘要**：研究利用重组CABP4蛋白证明其与视网膜膜上的鸟苷酸环化酶(GC)存在钙依赖性结合，调控GC活性以维持光适应过程中的钙稳态。
(注：以上文献为示例，实际文献需通过学术数据库检索核实。)
Calcium-binding protein 4 (CABP4) is a member of the neuronal calcium-binding protein family, predominantly expressed in retinal photoreceptors and synaptic terminals. It plays a critical role in regulating calcium-dependent signal transduction, particularly in modulating synaptic transmission between photoreceptors and bipolar cells. Structurally, CABP4 contains EF-hand motifs that enable calcium ion binding, influencing its interaction with target proteins such as voltage-gated calcium channels. This interaction fine-tunes calcium homeostasis, essential for maintaining proper phototransduction and neural communication in the retina.
Mutations in the CABP4 gene are linked to autosomal recessive retinal disorders, including congenital cone-rod synaptic disorder and incomplete congenital stationary night blindness (CSNB). These conditions manifest as impaired vision, photophobia, and reduced electroretinogram responses, highlighting CABP4's role in visual signal processing. Recombinant human CABP4 protein, produced via bacterial or mammalian expression systems, is widely used in structural and functional studies to elucidate its molecular mechanisms. It aids in investigating calcium-dependent conformational changes, protein-protein interactions, and pathogenic mutations. Additionally, recombinant CABP4 serves as a tool for developing therapeutic strategies, such as gene therapy or small-molecule interventions, targeting retinal degenerative diseases. Its study bridges gaps in understanding calcium signaling in sensory neurons and offers potential avenues for treating vision-related pathologies.
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