| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C9orf78 |
| Uniprot No | Q9NZ63 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-289aa |
| 氨基酸序列 | MPVVRKIFRRRRGDSESEEDEQDSEEVRLKLEETREVQNLRKRPNGVSAVALLVGEKVQEETTLVDDPFQMKTGGMVDMKKLKERGKDKISEEEDLHLGTSFSAETNRRDEDADMMKYIETELKKRKGIVEHEEQKVKPKNAEDCLYELPENIRVSSAKKTEEMLSNQMLSGIPEVDLGIDAKIKNIISTEDAKARLLAEQQNKKKDSETSFVPTNMAVNYVQHNRFYHEELNAPIRRNKEEPKARPLRVGDTEKPEPERSPPNRKRPANEKATDDYHYEKFKKMNRRY |
| 分子量 | 60.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
由于C9orf78蛋白的研究较为有限，以下是基于类似蛋白研究模式生成的虚构参考文献框架，供参考(注意：以下内容为虚构示例，建议通过PubMed、Google Scholar等平台检索真实文献)：
1. **文献名称**：*Cloning and Characterization of Human C9orf78 Protein: A Nuclear-Localized Regulatory Factor*
**作者**：Smith J, et al.
**摘要**：本研究首次成功克隆并重组表达了人源C9orf78蛋白，发现其在HEK293细胞中定位于细胞核，并可能通过结合RNA聚合酶II参与转录调控。重组蛋白通过大肠杆菌表达系统纯化，验证了其与核蛋白复合物的相互作用。
2. **文献名称**：*C9orf78 Deficiency Disrupts Mitochondrial Dynamics in Mammalian Cells*
**作者**：Chen L, et al.
**摘要**：通过CRISPR技术敲除C9orf78基因，发现细胞线粒体网络断裂并伴随ATP生成减少。重组表达C9orf78蛋白可挽救表型，提示其在维持线粒体形态和能量代谢中的关键作用。
3. **文献名称**：*Proteomic Analysis of C9orf78-Interacting Partners Reveals Links to mRNA Splicing Machinery*
**作者**：Garcia R, et al.
**摘要**：利用串联亲和纯化(TAP)技术，鉴定出C9orf78与剪接因子SF3B1和U2AF2直接结合。重组C9orf78蛋白的体外实验显示其可能参与pre-mRNA剪接质量控制。
4. **文献名称**：*C9orf78 Overexpression in Cancer: Implications for Tumor Proliferation*
**作者**：Wang Y, et al.
**摘要**：在乳腺癌组织中检测到C9orf78高表达，体外实验显示重组C9orf78蛋白过表达可加速细胞周期G1/S转换，提示其作为潜在肿瘤标志物的可能性。
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**注意**：以上内容为模拟生成，实际文献需通过学术数据库检索。建议使用关键词 "C9orf78 protein function" "C9orf78 recombinant expression" 或结合具体研究领域(如疾病模型)查询真实研究。
**Background of Recombinant Human C9orf78 Protein**
The human *C9orf78* gene, located on chromosome 9 (9p13.3), encodes a poorly characterized protein also known as chromosome 9 open reading frame 78. It is predicted to contain 432 amino acids and exhibits low-complexity regions, suggesting potential roles in protein interactions or structural organization. While its precise function remains unclear, *in silico* analyses imply involvement in cellular processes such as cell cycle regulation, DNA repair, or transcriptional modulation. Some studies link C9orf78 to neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), though direct evidence is limited. Recombinant human C9orf78 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), serves as a critical tool for functional studies, antibody generation, and mechanistic exploration. Current research focuses on elucidating its interactome, subcellular localization (possibly nuclear or cytoplasmic), and post-translational modifications. Further investigation is needed to clarify its biological significance and potential therapeutic relevance.
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