| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C9orf64 |
| Uniprot No | Q5T6V5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-160aa |
| 氨基酸序列 | MHLVVESFPSYRDVTLFEGKRVSFYKRAQILVADTWSVLEGKGDGCFKDISSITMFADYRLPQVLAHLGALKYSDDLLKKLLKGEMLSYGDRQEVEIRGCSLWCVELIRDCLLELIEQKGEKPNGEINSILLDYYLWDYAHDHREDMKGIPFHRIRCIYY |
| 分子量 | 45.1 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C9orf64蛋白的参考文献示例(**注：C9orf64研究较少，以下为模拟文献**)：
1. **文献名称**：*Characterization of Recombinant C9orf64 Protein and Its Putative Role in mRNA Splicing*
**作者**：Smith J, Doe R, Lee T.
**摘要**：研究通过原核表达系统纯化重组C9orf64蛋白，并发现其与剪接体复合物的相互作用，提示其在RNA剪接中的潜在作用。
2. **文献名称**：*C9orf64 Expression Profile and Structural Insights from Recombinant Protein Analysis*
**作者**：Chen X, Wang Y, Zhang L.
**摘要**：利用重组C9orf64进行结构预测和免疫印迹分析，发现其在睾丸组织高表达，并具有保守的α-螺旋结构域。
3. **文献名称**：*Functional Screening Links C9orf64 to Cellular Stress Response Pathways*
**作者**：Kumar A, Patel S.
**摘要**：通过体外重组蛋白实验，发现C9orf64与热休克蛋白HSP70相互作用，可能在细胞应激调控中起作用。
4. **文献名称**：*Bioinformatic and Recombinant-Based Study on C9orf64 Evolutionary Conservation*
**作者**：Garcia M, et al.
**摘要**：结合生物信息学与重组蛋白功能实验，揭示C9orf64在脊椎动物中的进化保守性及其可能的核酸结合能力。
**注意**：上述文献为模拟内容，实际研究中关于C9orf64的报道仍较少，建议通过数据库(如PubMed、UniProt)进一步核实最新进展。
The recombinant human C9orf64 protein is derived from the C9orf64 gene (Chromosome 9 Open Reading Frame 64), a poorly characterized gene located on chromosome 9p13.3. This gene encodes a protein of unknown function, with limited experimental data available in public databases. Bioinformatic analyses suggest that C9orf64 may contain conserved phosphorylation sites and intrinsically disordered regions, hinting at potential roles in cellular signaling or protein interactions. However, its exact molecular mechanisms and physiological relevance remain largely unexplored.
Interest in C9orf64 has emerged indirectly due to its chromosomal proximity to genes linked to diseases such as glioma and lung cancer, though no direct pathogenic associations have been confirmed. Recombinant C9orf64 protein is typically produced in heterologous expression systems (e.g., E. coli or mammalian cells) to enable functional studies, antibody production, or structural characterization. Recent transcriptomic datasets indicate variable C9orf64 expression across tissues, with higher levels detected in testis, brain, and certain cancer cell lines, suggesting tissue-specific roles.
Notably, the C9orf64 locus overlaps with regions linked to neurodevelopmental disorders, though no causative mutations have been identified. Current research focuses on elucidating its interactome and regulatory networks using recombinant protein tools. Challenges include the lack of validated antibodies and animal models, underscoring the need for further investigation into its biochemical properties and potential involvement in cellular processes or disease pathways.
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