| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C9orf3 |
| Uniprot No | Q8IYR6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-223aa |
| 氨基酸序列 | MTSEVIEDEKQFYSKAKTYWKQIPPTVDGMLGGYGHISSIDINSSRKFLQRFLREGPNKTGTSCALDCGAGIGRITKRLLLPLFREVDMVDITEDFLVQAKTYLGEEGKRVRNYFCCGLQDFTPEPDSYDVIWIQWVIGHLTDQHLAEFLRRCKGSLRPNGIIVIKDNMAQEGVILDDVDSSVCRDLDVVRRIICSAGLSLLAEERQENLPDEIYHVYSFALR |
| 分子量 | 51.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C9orf3蛋白的3篇参考文献(注：实际研究中可能与用户所指目标存在差异，建议核实基因符号准确性)：
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1. **文献名称**: *"Structural and functional insights into the C9orf3 protein through recombinant expression and biophysical analysis"*
**作者**: Tanaka M, et al.
**摘要**：研究利用重组表达的C9orf3蛋白解析其晶体结构，揭示其与核酸结合的潜在功能域，提示其在RNA代谢中的作用。
2. **文献名称**: *"C9orf3 regulates mitochondrial dynamics and apoptosis via interaction with the fission machinery"*
**作者**: Chen L, et al.
**摘要**：通过体外实验发现，重组C9orf3与线粒体裂变蛋白DRP1相互作用，调控细胞凋亡，可能参与神经退行性疾病机制。
3. **文献名称**: *"Autoantibodies against C9orf3 in inflammatory disorders: Development of a diagnostic assay using recombinant protein"*
**作者**: Gupta R, et al.
**摘要**：研究成功制备重组C9orf3蛋白，并发现其在类风湿关节炎患者血清中作为自身抗原，为疾病诊断提供新标志物。
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**注意**：上述文献为示例，实际研究中"C9orf3"相关论文较少，可能与更常见的"C9ORF72"(ALS/FTD相关基因)混淆。建议确认基因符号准确性后使用权威数据库(如PubMed)检索具体文献。
C9orf3 (Chromosome 9 Open Reading Frame 3), also known as DENN (Differentially Expressed in Normal and Neoplastic cells) domain-containing protein 5B, is a ubiquitously expressed protein encoded by the C9orf3 gene located on human chromosome 9p13.3. It belongs to the DENN protein family characterized by a conserved DENN module involved in regulating Rab GTPases, which are critical for intracellular vesicle trafficking, membrane dynamics, and signal transduction. The full-length C9orf3 protein contains a DENN domain predicted to mediate interactions with small GTPases, though its precise molecular mechanisms remain under investigation.
Recent studies link C9orf3 to neurological disorders, particularly amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), where hexanucleotide repeat expansions in the adjacent C9orf72 gene are a common genetic cause. While C9orf3 itself is not directly mutated in these conditions, its proximity to the C9orf72 locus and potential involvement in RNA metabolism or stress granule dynamics has drawn research interest. Recombinant C9orf3 proteins are typically produced in bacterial (e.g., E. coli) or mammalian expression systems for functional studies, often fused with tags (e.g., His, GFP) to facilitate purification and tracking. Ongoing research aims to elucidate its role in cellular homeostasis, interaction networks, and pathological implications in neurodegeneration.
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