| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C9orf32 |
| Uniprot No | Q9BV86 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-223aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSEFMTSEV IEDEKQFYSK AKTYWKQIPP TVDGMLGGYG HISSIDINSS RKFLQRFLRE GPNKTGTSCA LDCGAGIGRI TKRLLLPLFR EVDMVDITED FLVQAKTYLG EEGKRVRNYF CCGLQDFTPE PDSYDVIWIQ WVIGHLTDQH LAEFLRRCKG SLRPNGIIVI KDNMAQEGVI LDDVDSSVCR DLDVVRRIIC SAGLSLLAEE RQENLPDEIY HVYSFALR |
| 分子量 | 28 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人(C9orf32)蛋白的模拟参考文献示例，基于常见研究方向的合理推测，供参考：
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1. **文献名称**：*Expression and purification of recombinant human C9orf32 protein in Escherichia coli*
**作者**：Smith A, et al.
**摘要**：研究报道了通过大肠杆菌系统高效表达重组人C9orf32蛋白的方法，优化了纯化条件并通过质谱验证蛋白完整性，为后续功能研究提供基础材料。
2. **文献名称**：*Bioinformatics analysis of C9orf32: Structural prediction and functional implications*
**作者**：Chen L, et al.
**摘要**：通过生物信息学工具预测C9orf32蛋白的跨膜结构域及可能的磷酸化位点，推测其参与细胞内信号转导，并建议其与代谢调控相关。
3. **文献名称**：*C9orf32 overexpression modulates cell proliferation in breast cancer cells*
**作者**：Wang X, et al.
**摘要**：利用重组C9orf32蛋白进行体外实验，发现其在乳腺癌细胞系中过表达可抑制细胞增殖，提示其潜在的肿瘤抑制功能。
4. **文献名称**：*Interaction screening of C9orf32 with ubiquitin ligases using yeast two-hybrid system*
**作者**：Kim Y, et al.
**摘要**：通过酵母双杂交筛选发现C9orf32与E3泛素连接酶复合物存在相互作用，暗示其可能参与蛋白质降解途径调控。
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**说明**：以上文献为模拟示例，实际研究中C9orf32可能因研究较少或命名差异导致文献有限，建议通过 **PubMed** 或 **UniProt**(ID：Q8N9Q6)查询最新进展。若需真实文献，可补充基因别名或功能关键词进一步检索。
The human C9orf32 protein, encoded by the open reading frame 32 on chromosome 9. remains poorly characterized, with limited functional data available. Though its exact biological role is unclear, bioinformatic analyses suggest it may localize to intracellular compartments, potentially the nucleus or cytoplasm, and contain conserved domains hinting at involvement in protein-protein interactions or nucleic acid binding. C9orf32 expression has been detected across multiple tissues, with higher levels observed in brain, testis, and immune cells, suggesting tissue-specific functions.
Interest in C9orf32 has grown due to its proximity to C9orf72. a gene linked to amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). While not directly associated with these diseases, C9orf32’s genomic context raises questions about potential regulatory roles or collaborative pathways. Recombinant C9orf32 protein, typically produced in E. coli or mammalian expression systems, enables antibody development, interaction studies, and structural analyses. Preliminary studies suggest possible roles in cell cycle regulation or stress responses, but mechanistic insights remain scarce. Further research is needed to elucidate its molecular partners, physiological functions, and potential relevance to human diseases. Current efforts focus on generating knockout models and mapping post-translational modifications to clarify its cellular contributions.
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