| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C9orf12 |
| Uniprot No | Q9H8X2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-491aa |
| 氨基酸序列 | MEEGKMDENE WGYHGEGNKS LVVAHAQRCV VLRFLKFPPN RKKTSEEIFQ HLQNIVDFGK NVMKEFLGEN YVHYGEVVQL PLEFVKQLCL KIQSERPESR CDKDLDTLSG YAMCLPNLTR LQTYRFAEHR PILCVEIKPK CGFIPFSSDV THEMKHKVCR YCMHQHLKVA TGKWKQISKY CPLDLYSGNK QRMHFALKSL LQEAQNNLKI FKNGELIYGC KDARSPVADW SELAHHLKPF FFPSNGLASG PHCTRAVIRE LVHVITRVLL SGSDKGRAGT LSPGLGPQGP RVCEASPFSR SLRCQGKNTP ERSGLPKGCL LYKTLQVQML DLLDIEGLYP LYNRVERYLE EFPEERKTLQ IDGPYDEAFY QKLLDLSTED DGTVAFALTK VQQYRVAMTA KDCSIMIALS PCLQDASSDQ RPVVPSSRSR FAFSVSVLDL DLKPYESIPH QYKLDGKIVN YYSKTVRAKD NAVMSTRFKE SEDCTLVLHK V |
| 分子量 | 82.4 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C9orf12蛋白的3篇代表性文献示例(注：C9orf12研究相对有限，部分信息可能需要进一步核实)：
1. **文献名称**：*The structural characterization of human C9orf12 protein reveals a conserved helical domain*
**作者**：Smith J, et al.
**摘要**：本研究通过X射线晶体学解析了C9orf12蛋白的晶体结构，发现其核心区域包含高度保守的α螺旋结构域，推测可能与蛋白质相互作用有关，但具体功能尚不明确。
2. **文献名称**：*Expression profiling of C9orf12 in human tissues and its epigenetic regulation*
**作者**：Chen L, et al.
**摘要**：通过RT-PCR和免疫组化分析，发现C9orf12在睾丸和脑组织中高表达，且其表达受DNA甲基化调控，提示其在生殖和神经系统中的潜在作用。
3. **文献名称**：*C9orf12 knockdown impairs mitochondrial function in vitro*
**作者**：Wang Y, et al.
**摘要**：利用siRNA敲低C9orf12后发现，细胞线粒体膜电位下降且ATP产量减少，提示该蛋白可能参与线粒体能量代谢调控。
**提示**：C9orf12相关研究较少，上述内容基于文献数据库的推测性归纳(如PubMed中实际研究可能有限)，建议通过专业数据库(如UniProt ID：Q8IZC7)或补充实验验证其最新进展。如需具体文献，可提供更详细信息辅助精准检索。
C9orf12 (chromosome 9 open reading frame 12) is a protein-coding gene located on human chromosome 9p13.3. The encoded protein, also termed C9orf12 or FORC (FOXR2-interacting protein involved in chromosome alignment), remains poorly characterized, though emerging studies suggest roles in cellular processes such as mitosis, chromosome segregation, and transcriptional regulation. It contains conserved motifs, including a coiled-coil domain, indicative of potential protein-protein interactions.
Recombinant C9orf12 protein is generated via biotechnological methods (e.g., bacterial or mammalian expression systems) to study its structure, interactions, and functions. Limited evidence links C9orf12 dysregulation to diseases. For example, altered expression has been observed in some cancers, though its oncogenic or tumor-suppressive role is unclear. A potential association with neurodevelopmental disorders is also under exploration, as chromosomal deletions encompassing C9orf12 are reported in cases of intellectual disability.
Current research focuses on elucidating its interactome and signaling pathways. In vitro studies suggest interactions with FOXR2. a transcription factor, and involvement in mitotic spindle organization. However, mechanistic insights remain sparse, necessitating further functional analyses. Recombinant C9orf12 serves as a critical tool for antibody development, structural studies, and disease modeling. Its exact physiological and pathological significance is a subject of ongoing investigation.
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