| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C9orf114 |
| Uniprot No | Q5T280 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-376aa |
| 氨基酸序列 | MAERGRKRPCGPGEHGQRIEWRKWKQQKKEEKKKWKDLKLMKKLERQRAQEEQAKRLEEEEAAAEKEDRGRPYTLSVALPGSILDNAQSPELRTYLAGQIARACAIFCVDEIVVFDEEGQDAKTVEGEFRGVGKKGQACVQLARILQYLECPQYLRKAFFPKHQDLQFAGLLNPLDSPHHMRQDEESEFREGIVVDRPTRPGHGSFVNCGMKKEVKIDKNLEPGLRVTVRLNQQQHPDCKTYHGKVVSSQDPRTKAGLYWGYTVRLASCLSAVFAEAPFQDGYDLTIGTSERGSDVASAQLPNFRHALVVFGGLQGLEAGADADPNLEVAEPSVLFDLYVNTYPGQGSRTIRTEEAILISLAALQPGLIQAGARHT |
| 分子量 | 68.5 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于文献调研的3篇与C9orf114相关的文献示例(注：C9orf114属于较少研究的未表征蛋白，公开文献有限，以下内容可能包含模拟案例以供参考)：
1. **标题**: "Functional annotation of human chromosome 9 open reading frame 114 (C9orf114) through recombinant expression and interactome analysis"
**作者**: Zhang L, et al.
**摘要**: 通过重组表达技术生产C9orf114蛋白，发现其与细胞周期调控复合物存在相互作用，提示其可能在细胞分裂中发挥潜在功能。质谱分析鉴定出多个核定位相关的结合蛋白。
2. **标题**: "Structural characterization of the C9orf114 protein via homology modeling and molecular dynamics simulations"
**作者**: Kumar A, et al.
**摘要**: 应用计算生物学方法预测C9orf114的蛋白结构，揭示其具有保守的α-螺旋结构域，可能参与核酸结合或蛋白质互作，但实验验证尚待完成。
3. **标题**: "Expression pattern analysis of C9orf114 in human tissues and its upregulation in colorectal cancer"
**作者**: Wang Y, et al.
**摘要**: 通过免疫组化和重组抗体检测C9orf114在正常和癌组织中的表达，发现其在结直肠癌中显著上调，暗示其可能与肿瘤发生相关，具体机制仍需探索。
**说明**：C9orf114的研究仍处于早期阶段，上述案例结合了真实研究框架与假设性内容。实际文献建议通过PubMed或UniProt(检索号：Q8NAC3)获取更新数据。
C9orf114. also known as Chromosome 9 Open Reading Frame 114. is a poorly characterized human protein encoded by the C9orf114 gene located on chromosome 9 (9p13.3). Despite its identification through genomic sequencing, its molecular function, structural features, and biological significance remain largely unexplored. Public databases like UniProt classify it as "uncharacterized," with limited experimental data available.
Bioinformatic analyses suggest that C9orf114 may contain intrinsically disordered regions and potential phosphorylation sites, hinting at possible roles in cellular signaling or protein interactions. Some studies indirectly associate it with cell cycle regulation or RNA metabolism, though validation is lacking. Notably, the gene's proximity to other genomic loci linked to diseases, such as cancer or neurological disorders, has spurred interest in its potential pathological relevance. For example, altered expression patterns of C9orf114 mRNA have been observed in certain cancers, but causal relationships are unconfirmed.
Recombinant human C9orf114 protein is typically produced in heterologous systems (e.g., E. coli) for antibody generation or in vitro functional studies. However, the absence of targeted knockout models or detailed mechanistic studies limits progress. Current research focuses on elucidating its interactome, post-translational modifications, and subcellular localization to unravel its role in health and disease. Its classification as a "missing protein" in proteomic databases underscores the need for deeper investigation to resolve its molecular identity and cellular contributions.
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