| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C8orf30A |
| Uniprot No | Q9BTY7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-390aa |
| 氨基酸序列 | MGEAGAGAGASGGPEASPEAEVVKLLPFLAPGARADLQAAAVRHVLALTGCGPGRALLAGQAALLQALMELAPASAPARDAARALVNLAADPGLHETLLAADPGLPARLMGRALDPQWPWAEEAAAALANLSREPAPCAALMAALAAAEPADSGLERLVRALCTPGYNARAPLHYLAPLLSNLSQRPAARAFLLDPDRCVVQRLLPLTQYPDSSVRRGGVVGTLRNCCFEHRHHEWLLGPEVDILPFLLLPLAGPEDFSEEEMERLPVDLQYLPPDKQREPDADIRKMLVEAIMLLTATAPGRQQVRDQGAYLILRELHSWEPEPDVRTACEKLIQVLIGDEPERGMENLLEVQVPEDVEQQLQQLDCREQEQLERELAPEPWVERATPT |
| 分子量 | 68.5 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C8orf30A蛋白的假设性参考文献示例(注：所列文献为示例，非真实存在)：
---
1. **文献名称**: *Characterization of recombinant human C8orf30A and its role in mitochondrial function*
**作者**: Smith J. et al. (2020)
**摘要**: 研究通过重组表达技术在大肠杆菌中制备了人C8orf30A蛋白，发现其定位于线粒体，并参与调节氧化磷酸化过程;敲除实验表明其缺失会导致ATP合成减少，提示其在能量代谢中的潜在作用。
2. **文献名称**: *C8orf30A interacts with the mTOR signaling pathway to regulate cell proliferation*
**作者**: Zhang L. et al. (2018)
**摘要**: 利用重组C8orf30A蛋白进行免疫共沉淀筛选，发现其与mTOR复合物亚基相互作用，体外实验显示过表达会抑制细胞增殖，推测其可能通过mTOR通路发挥肿瘤抑制功能。
3. **文献名称**: *Structural analysis of the human C8orf30A protein reveals a novel zinc-binding domain*
**作者**: Lee H. et al. (2019)
**摘要**: 通过重组蛋白结晶和X射线衍射技术解析了C8orf30A的晶体结构，发现其含有独特的锌结合域，可能参与DNA结合或蛋白互作，为功能研究提供结构基础。
4. **文献名称**: *Dysregulation of C8orf30A expression in inflammatory bowel disease*
**作者**: Brown K. et al. (2021)
**摘要**: 重组C8orf30A蛋白在体外实验中显著降低巨噬细胞炎症因子释放，临床样本分析显示其在炎症性肠病患者中表达异常，提示其抗炎治疗潜力。
---
建议通过PubMed或Google Scholar以**“C8orf30A recombinant”**或**“C8orf30A protein function”**为关键词检索真实文献。如需进一步协助定位实际研究，请告知。
**Background of Human Recombinant C8orf30A Protein**
The human *C8orf30A* (Chromosome 8 Open Reading Frame 30A) gene encodes a conserved protein of unknown molecular function, also referred to as C8orf30 or FLJ38471. It is annotated as a member of the DUF3523 protein family, characterized by a conserved domain of unknown function. Though poorly characterized, bioinformatic analyses suggest potential roles in metal ion binding or enzymatic activity due to structural homology with metalloproteins.
*C8orf30A* is broadly expressed across tissues, with elevated levels in the testes, implying a possible role in reproductive biology. Mouse studies indicate testis-specific expression of its ortholog, *Gm8989*, suggesting evolutionary conservation in germ cell development or spermatogenesis. Recombinant C8orf30A is produced to study its biochemical properties, interactions, and subcellular localization, often leveraging *in vitro* overexpression systems or tagged protein constructs.
While its physiological significance remains unclear, *C8orf30A* has been linked to cancer progression in bioinformatics screens, showing altered expression in gliomas and breast tumors. Its potential as a biomarker or therapeutic target is under exploration. Current research focuses on elucidating its molecular interactions, structural features, and involvement in cellular pathways like apoptosis or redox regulation. Further functional studies are needed to define its role in health and disease.
×
