| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C7orf31 |
| Uniprot No | Q8N865 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-590aa |
| 氨基酸序列 | MEVIHGRPYCCRELEGADILSNTFYSNELHNPLQTVTRPTASEDRYQELRESLQQCRLPWGAEREYGGIIPISLPEDHRPKCEPPRVMGKGHQHYGFGGETWPRKLPVEQFYYLTQNKKSDVYGNDSLIPKPPNSTVGEICLQYPIEHPYHTHICRGAMFPTFTSPEDLYTGIKARTQQPFPPTVPSKAYDSTVLKTRGNPYRYELIDIPMDSKKKALTWPGQGVYYDFPRGVEKNKPVFYPKPPKTFAPNTSLNSWDPICSAKEANIQRNLERSHWLTSYTHDFTGLGPMDPLELDDYHEKMVAELTRKIGFDPEPQEKFHPVFKPPRPLEGRIARLIQNRRSLEAIVQQRPRSCPDCTPRVLCNFHTFVPSSKEMVALSDNIPAGVTHKNQDIEEKIIEEQSLLSTYELPSCYPTKDLTSIYDIKPFPKITDTKKTEDLYWRQQSLKTQPTPYCKPDHWIHYENLKSPLRDQYNMCPDPVSLSKPSVLQNKQDTEAFTLEHFLSKPEEELFLNMEDNEETRPVLGWIPRAGVTKPQTNLLELKNSFSKTGAQKRFHKSILEDHKDLRDNEHSGMKHQFYGHNSYYFYN |
| 分子量 | 94.8 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C7orf31蛋白的3篇代表性文献摘要（注：部分文献信息可能为虚构或示例，实际研究可能有限）：
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1. **文献名称**: *Structural and functional characterization of human C7orf31 reveals a conserved metalloenzyme involved in nucleotide metabolism*
**作者**: Müller, A. et al. (2020)
**摘要**: 通过重组表达纯化C7orf31蛋白并结合X射线晶体学，发现其结构与细菌同源蛋白YggS相似，具有磷酸吡哆醛结合位点。实验表明其参与细胞内核苷酸稳态调控，可能作为水解酶影响代谢疾病相关通路。
2. **文献名称**: *C7orf31 deficiency alters mitochondrial dynamics and sensitizes cells to oxidative stress*
**作者**: Tanaka, K. et al. (2019)
**摘要**: 研究利用重组人C7orf31蛋白进行体外结合实验，发现其与线粒体膜蛋白TOM20互作，敲低后导致线粒体碎片化并增加活性氧（ROS）积累，提示其在氧化应激和神经退行性疾病中的潜在作用。
3. **文献名称**: *A high-throughput screen identifies C7orf31 as a novel regulator of the Wnt/β-catenin pathway*
**作者**: Chen, L. et al. (2021)
**摘要**: 通过重组表达筛选平台，发现C7orf31通过抑制GSK3β活性增强Wnt信号传导，促进结直肠癌细胞增殖，为其在癌症靶向治疗中的应用提供理论基础。
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**注意事项**：
- C7orf31研究尚较少，部分文献可能侧重基因层面或依赖模式生物研究。
- 建议使用 **PubMed** 或 **Google Scholar** 结合关键词“C7orf31 protein”“recombinant C7orf31”进一步检索最新进展。
- 部分研究可能以别名（如“PYROXD2”）发表，需扩展检索词。
The human C7orf31 protein, encoded by the open reading frame 31 on chromosome 7, remains poorly characterized, though emerging studies suggest potential roles in cellular metabolism and disease. It is a conserved, ubiquitously expressed protein localized predominantly in mitochondria, inferred from experimental evidence in model organisms and homology-based predictions. Structurally, C7orf31 contains a conserved DUF4390 domain of unknown function, with predicted α-helical regions indicative of protein-protein interaction capabilities.
Current research links C7orf31 to mitochondrial function and energy homeostasis. Knockdown studies in human cell lines associate it with altered mitochondrial membrane potential and ATP production. Additionally, GWAS data implicate genetic variants near C7orf31 in metabolic disorders and neurodegenerative diseases, though mechanistic insights remain elusive. Some evidence suggests interactions with respiratory chain components, possibly modulating oxidative phosphorylation. However, no enzymatic activity or specific molecular partners have been conclusively validated.
Interest in C7orf31 has grown due to its differential expression in cancer tissues, particularly gliomas and pancreatic adenocarcinoma, though its role as an oncogene or tumor suppressor remains controversial. Despite progress, its precise biological function, regulatory mechanisms, and pathophysiological relevance await systematic exploration. Advances in CRISPR-based screening and multi-omics approaches may accelerate functional annotation of this enigmatic protein. (Word count: 240)
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