| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C7orf30 |
| Uniprot No | Q96EH3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-234aa |
| 氨基酸序列 | MGPGGRVARL LAPLMWRRAV SSVAGSAVGA EPGLRLLAVQ RLPVGAAFCR ACQTPNFVRG LHSEPGLEER AEGTVNEGRP ESDAADHTGP KFDIDMMVSL LRQENARDIC VIQVPPEMRY TDYFVIVSGT STRHLHAMAF YVVKMYKHLK CKRDPHVKIE GKDTDDWLCV DFGSMVIHLM LPETREIYEL EKLWTLRSYD DQLAQIAPET VPEDFILGIE DDTSSVTPVE LKCE |
| 分子量 | 52.6 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **文献名称**: "Functional characterization of C7orf30 in mitochondrial ribosome assembly"
**作者**: Smith A, et al.
**摘要**: 研究发现C7orf30（后称MTRG）通过与线粒体核糖体亚基结合，参与线粒体RNA加工和核糖体组装，缺失导致线粒体呼吸链功能异常。
2. **文献名称**: "C7orf30 links METTL8-mediated tRNA modification to oxidative phosphorylation"
**作者**: Li J, et al.
**摘要**: 揭示C7orf30与甲基转移酶METTL8互作，调控线粒体tRNA修饰，进而影响氧化磷酸化效率和细胞能量代谢稳态。
3. **文献名称**: "C7orf30 as a potential tumor suppressor in colorectal cancer"
**作者**: Wang Y, et al.
**摘要**: 通过组织芯片分析，发现C7orf30在结直肠癌中表达显著下调，过表达抑制癌细胞增殖并诱导凋亡，提示其肿瘤抑制功能。
4. **文献名称**: "Structural insights into C7orf30’s role in RNA-protein complex stabilization"
**作者**: Chen X, et al.
**摘要**: 利用冷冻电镜解析C7orf30与核糖体RNA复合物结构，阐明其通过结合RNA螺旋域稳定复合物构象，可能参与翻译调控。
注：上述文献信息为模拟生成，实际需根据具体研究查阅真实文献。
The human C7orf30 protein, encoded by the C7orf30 gene on chromosome 7q, is a conserved but poorly characterized protein implicated in mitochondrial function and cellular energy metabolism. Initially identified through genomic studies, its exact molecular mechanisms remain elusive. Orthologs in model organisms, such as yeast Ydr165c and mouse C7orf30, suggest evolutionary conservation in eukaryotes. Recent studies propose its potential role as a subunit of the mitochondrial intermediate peptidase (MIPEP) complex, which processes nuclear-encoded mitochondrial precursor proteins to support organelle homeostasis.
C7orf30 is ubiquitously expressed, with elevated levels in energy-demanding tissues like the heart, brain, and skeletal muscle. Though structurally unclassified, predictions indicate possible alpha-helical domains and nuclear localization signals. Dysregulation of C7orf30 has been loosely associated with cancers, cardiovascular disorders, and neurodegenerative diseases, though causal links require validation. In vitro experiments show its interaction with mitochondrial respiratory chain components, hinting at roles in oxidative phosphorylation or stress response.
Current research focuses on resolving its 3D structure, precise enzymatic functions, and disease-related pathways. CRISPR-based screening and proteomic approaches aim to clarify its interactome and regulatory networks. Despite limited understanding, C7orf30's mitochondrial ties and conservation underscore its biological significance, warranting deeper exploration in cellular metabolism and pathology. (299 words)
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