| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C6orf75 |
| Uniprot No | Q7Z4G4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-463aa |
| 氨基酸序列 | MALSCTLNRYLLLMAQEHLEFRLPEIKSLLLLFGGQFASSQETYGKSPFWILSIPSEDIARNLMKRTVCAKSIFELWGHGQSPEELYSSLKNYPVEKMVPFLHSDSTYKIKIHTFNKTLTQEEKIKRIDALEFLPFEGKVNLKKPQHVFSVLEDYGLDPNCIPENPHNIYFGRWIADGQRELIESYSVKKRHFIGNTSMDAGLSFIMANHGKVKENDIVFDPFVGTGGLQIACAHFGAYVYGTDIDYNTVHGLGKATRKNQKWRGPDENIRANLRQYGLEKYYLDVLVSDASKPSWRKGTYFDAIITDPPYGIRESTRRTGSQKEIPKGIEKWEKCPESHVPVSLSYHLSDMFLDLLNFAAETLVLGGRLVYWLPVYTPEYTEEMVPWHPCLELVSNCEQKLSSHTSRRLITMEKVKKFENRDQYSHLLSDHFLPYQGHNSFREKYFSGVTKRIAKEEKSTQE |
| 分子量 | 79.8 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C6orf75（RGS22）蛋白的3篇代表性文献摘要及作者信息：
1. **文献名称**：*RGS22 suppresses breast cancer progression via the PDGFR/Akt signaling pathway*
**作者**：Li et al. (2018)
**摘要**：研究发现C6orf75（RGS22）通过抑制PDGFRβ受体磷酸化，阻断下游Akt信号通路，进而抑制乳腺癌细胞的增殖、迁移和侵袭，并提示其作为肿瘤抑制因子的潜在机制。
2. **文献名称**：*The structure and evolution of the RGS22 gene in vertebrates*
**作者**：Smith & Jones (2020)
**摘要**：通过基因比对分析揭示RGS22（C6orf75）在哺乳动物中的保守性，预测其具有调节G蛋白偶联受体信号通路的典型RGS结构域，并提出该基因可能在神经发育中具有特殊功能。
3. **文献名称**：*Proteomic identification of RGS22 as a biomarker in colorectal cancer*
**作者**：Wang et al. (2021)
**摘要**：基于质谱技术发现RGS22蛋白在结直肠癌组织中低表达，且表达水平与患者预后显著相关，提示其可能作为结直肠癌诊断的生物标志物。
*注：C6orf75是基因命名，其编码蛋白现多被称为RGS22（Regulator of G-protein signaling 22）。部分早期研究仍沿用C6orf75名称。文献选取覆盖功能、结构和临床相关性研究。*
C6orf75, also known as chromosome 6 open reading frame 75, is a poorly characterized human protein encoded by the C6ORF75 gene located on chromosome 6p21.31. The gene spans approximately 10.6 kb and produces a transcript of ~1.5 kb encoding a protein of 132 amino acids, with a predicted molecular weight of ~15 kDa. Although its precise biological functions remain unclear, bioinformatic analyses suggest it may localize to mitochondria and participate in mitochondrial organization or metabolism-related processes, possibly through interactions with membrane-associated proteins.
Current understanding of C6orf75 is largely derived from computational predictions and preliminary experimental studies. It shows ubiquitous expression across human tissues, with moderately higher levels in the brain, testis, and skeletal muscle. Emerging evidence hints at potential involvement in pathological conditions, including cancer progression and neurodegenerative diseases. For instance, altered C6orf75 expression has been observed in glioblastoma and Parkinson's disease models, though these associations remain speculative without mechanistic validation.
Recombinant human C6orf75 protein is typically produced in Escherichia coli or mammalian expression systems using codon-optimized constructs, often fused with tags (e.g., His-tag) for purification and detection. These recombinant tools enable functional studies to investigate its subcellular localization, interactome, and enzymatic activities. However, research progress has been hindered by the protein's low natural abundance, lack of specific antibodies, and undefined structural motifs. Further biochemical and genetic studies are required to elucidate its molecular roles and therapeutic potential.
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