| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C6orf165 |
| Uniprot No | Q8IYR0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-622aa |
| 氨基酸序列 | MPPTQAESVIRSIIREIGQECAAHGEIVSETLIAFMVKAVVLDPSNGFNMDRTLMKSDVQNLVKLCMTRLLDTKNPSLDTIKMQVYFDMNYTNRVEFLEEHHRVLESRLGSVTREITDNRACAKEELESLYRKIISYVLLRSGLGSPTDIKTVREVTAALQSVFPQAELGTFLTLSKKDKERQLKELTMIVTGIRLFNRDCGKGGEGIDDLPAVLHVAIPATMQHIDYQLETARSQVYRYTAILEKAANDPLMRAELQPYMLKEALYNIRQYEVFLQIILSDIITGAQEVEMMTKQLGAHLEQLKMTIKSKIAVPTSQVFPIFIALSTLWTSLQDETIVVGVLSNLFTHIQPFLGAHELYFPERVMQCHLNGATVKTDVCRMKEHMEDRVNVADFRKLEWLFPETTANFDKLLIQYRGFCAYTFAATDGLLLPGNPAIGILKYKEKYYTFNSKDAAYSFAENPEHYIDIVREKAKKNTELIQLLELHQQFETFIPYSQMRDADKHYIKPITKCESSTQTNTHILPPTIVRSYEWNEWELRRKAIKLANLRQKVTHSVQTDLSHLRRENCSQVYPPKDTSTQSMREDSTGVPRPQIYLAGLRGGKSEITDEVKVNLTRDVDET |
| 分子量 | 97.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C6orf165蛋白的假设性参考文献示例（非真实文献，仅作参考格式示范）：
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1. **文献名称**: *Characterization of the Human C6orf165 Protein: Expression, Localization, and Functional Analysis*
**作者**: Smith A, et al.
**摘要**: 研究首次在大肠杆菌中重组表达并纯化C6orf165蛋白，发现其定位于细胞线粒体，并通过酵母双杂交筛选发现其与代谢相关蛋白相互作用，提示其可能参与能量调控。
2. **文献名称**: *C6orf165 Encodes a Novel Stress-Responsive Protein in Human Cells*
**作者**: Lee J, Kim H.
**摘要**: 报道C6orf165在哺乳动物细胞中的重组表达，发现其转录水平在氧化应激条件下显著上调，并发现其通过激活MAPK通路调节细胞凋亡。
3. **文献名称**: *Structural Prediction and Interaction Partners of C6orf165: A Bioinformatics Approach*
**作者**: Zhang Y, et al.
**摘要**: 结合生物信息学与体外实验，预测C6orf165蛋白的α-螺旋结构域，并利用重组蛋白进行Pull-down实验，鉴定出与RNA结合蛋白的潜在相互作用，暗示其可能参与RNA代谢。
4. **文献名称**: *Functional Study of C6orf165 in Cancer Cell Proliferation*
**作者**: Rossi M, et al.
**摘要**: 通过重组表达C6orf165蛋白的截短突变体，发现其抑制肿瘤细胞增殖的能力依赖于N端结构域，可能与抑制Wnt信号通路相关。
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**注意**：以上文献为示例，实际研究中C6orf165的功能可能尚不明确。
**建议**：通过NCBI PubMed或UniProt数据库（检索号：Q8N9W9）获取最新研究进展，并结合关键词如“C6orf165 recombinant protein”进一步筛选。
**Background of Recombinant Human C6orf165 Protein**
The C6orf165 gene, located on chromosome 6 (6q24.3), encodes a protein of unknown function, also referred to as chromosome 6 open reading frame 165. While its precise biological role remains poorly characterized, bioinformatic analyses suggest that C6orf165 may harbor conserved domains associated with enzymatic or structural activities, potentially linked to nucleic acid binding or protein interactions. The protein is evolutionarily conserved across vertebrates, with homologs identified in model organisms, implying functional importance.
Recombinant human C6orf165 protein is typically produced in heterologous expression systems (e.g., *E. coli* or mammalian cells*) for functional studies. Its recombinant form enables investigations into molecular interactions, subcellular localization, and potential involvement in cellular pathways such as stress response or genome maintenance. Despite limited experimental data, transcriptomic studies suggest tissue-specific expression patterns, with higher levels observed in testes, brain, and immune cells, hinting at roles in specialized physiological processes.
Current research focuses on elucidating its molecular mechanisms, with hypotheses suggesting ties to DNA repair, RNA metabolism, or mitochondrial function. However, the absence of detailed structural or biochemical data underscores the need for further characterization. Recombinant C6orf165 serves as a critical tool for probing its contributions to cellular homeostasis and disease contexts, particularly cancer or neurodegenerative disorders, where dysregulation of conserved but unannotated genes is increasingly implicated.
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