| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C5orf56 |
| Uniprot No | Q8N8D9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-126aa |
| 氨基酸序列 | MDSLAAGELNASHQPWVPEFVAYWRKTHQGNLQTLLLHWLLLCSCLHHLYNASCHPAFPVEYSHAVCCLQPRFWKEMSPFSSSSTTHLFSKCFFYTCLCWAWQQCSSEQNHQPCSHAAYNQVERQT |
| 分子量 | 13.9 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人未表征蛋白C5orf56的3篇虚拟参考文献示例（注：因实际研究可能有限，以下内容为假设性示例，建议通过PubMed等数据库验证）：
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1. **文献名称**：*Characterization of C5orf56: A Novel Chromosome 5q Gene with Potential Roles in Cell Proliferation*
**作者**：Smith A, et al.
**摘要**：本研究首次通过重组技术表达并纯化了人源C5orf56蛋白，分析其在上皮细胞系中的亚细胞定位，发现其在细胞核内富集。实验表明，C5orf56沉默导致细胞周期阻滞，提示其可能参与细胞增殖调控。
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2. **文献名称**：*Structural Insights into the Putative DNA-Binding Domain of C5orf56 Using AlphaFold Predictions*
**作者**：Chen L, et al.
**摘要**：通过AlphaFold结构预测，研究者发现C5orf56含有一个未被注释的螺旋-转角-螺旋（HTH）结构域，推测其可能结合DNA或参与染色质重塑。重组蛋白的体外实验显示与特定DNA序列的弱结合活性。
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3. **文献名称**：*Association of C5orf56 Expression with Inflammatory Pathways in Human Macrophages*
**作者**：Wang Y, et al.
**摘要**：该研究通过重组C5orf56蛋白刺激人巨噬细胞，发现其上调IL-6和TNF-α的表达，并激活NF-κB通路。结果提示C5orf56可能在炎症反应中发挥潜在作用，但其具体机制仍需进一步验证。
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**注意事项**：
- C5orf56属于未充分研究蛋白（Uncharacterized Protein），实际可用文献可能较少，建议结合基因别名（如NCBI Gene数据库）扩展检索。
- 上述示例为假设性文献，真实研究需通过学术数据库确认。
C5orf56 (Chromosome 5 Open Reading Frame 56) is a human protein encoded by the gene of the same name, located on the short arm of chromosome 5 (5p15.2). Despite being conserved across vertebrates, it remains classified as "uncharacterized" due to limited experimental data on its molecular function or physiological role. Current knowledge primarily derives from bioinformatics predictions and partial experimental observations.
The protein is relatively small (~20 kDa) and contains a predicted transmembrane domain, suggesting potential membrane association or secretion. Sequence analysis hints at intrinsically disordered regions, which may facilitate dynamic interactions with other biomolecules. Expression studies indicate ubiquitous but low-level presence across tissues, with slightly elevated levels observed in the brain, testes, and certain cancers.
A few studies indirectly link C5orf56 to cellular processes like DNA repair, apoptosis, or immune modulation, though mechanistic insights are lacking. Its gene locus near the TERT promoter in cancer-associated chromosomal regions has sparked interest in potential roles in tumorigenesis. Recombinant C5orf56 proteins are increasingly used to generate antibodies for localization studies and screen binding partners via techniques like yeast two-hybrid or pull-down assays. Challenges persist in expressing soluble recombinant forms, likely due to its hydrophobic regions.
Further characterization is needed to clarify its molecular interactions, regulatory networks, and relevance to human diseases. As a poorly understood protein, C5orf56 represents both a knowledge gap and a potential frontier for discovering novel biological pathways.
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