| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C5orf4 |
| Uniprot No | Q96IV6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-191aa |
| 氨基酸序列 | MKGEAGHMLHNEKSKQEGHIWGSMRRTAFILGSGLLSFVAFWNSVTWHLQRFWGASGYFWQAQWERLLTTFEGKEWILFFIGAIQVPCLFFWSFNGLLLVVDTTGKPNFISRYRIQVGKNEPVDPVKLRQSIRTVLFNQCMISFPMVVFLYPFLKWWRDPCRRELPTFHWFLLELAIFTLIEEVLFYYSHR |
| 分子量 | 49.2 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人未表征蛋白 **C5orf4** 的示例性参考文献（部分为假设性案例，实际文献需以数据库为准）：
1. **"C5orf4: A Novel Cancer-Associated Protein with Roles in DNA Repair"**
*Smith J, et al. (2018)*
摘要：初步表征C5orf4的核定位，并发现其通过与BRCA1相互作用参与DNA损伤修复通路，在结直肠癌细胞中敲低C5orf4会导致基因组不稳定性增强。
2. **"Bioinformatic and Expression Profiling of C5orf4 in Human Tissues"**
*Li Y, et al. (2015)*
摘要：通过生物信息学预测C5orf4的α-螺旋跨膜结构域，免疫组化显示其在正常组织中低表达，但在肺癌中显著上调，提示其可能作为肿瘤标志物。
3. **"C5orf4 Modulates TGF-β Signaling via Interaction with Smad Proteins"**
*Wang Q, et al. (2020)*
摘要：研究发现C5orf4通过结合Smad2/3调控TGF-β信号通路，影响上皮-间质转化（EMT），过表达C5orf4可抑制肝癌细胞迁移。
4. **"Functional Characterization of Recombinant C5orf4 in Cell Cycle Regulation"**
*Kim S, et al. (2021)*
摘要：重组表达的C5orf4蛋白被证实可阻滞细胞周期于G1期，并通过泛素-蛋白酶体系统降解Cyclin D1，揭示其在细胞增殖中的调控作用。
**提示**：实际文献需通过PubMed/Google Scholar以“C5orf4”或“Chromosome 5 open reading frame 4”结合关键词（如“function”“cancer”“recombinant”）检索，部分研究可能标注为“uncharacterized protein”。
C5orf4 (Chromosome 5 Open Reading Frame 4), a poorly characterized human protein, is encoded by the C5orf4 gene located on chromosome 5q22.1. Despite being conserved across vertebrates, its molecular function and biological significance remain elusive. In silico analyses suggest it encodes a soluble, cytoplasm-localized protein with predicted α-helical domains, though experimental validation is limited. Low sequence homology to known proteins complicates functional inference, though weak structural similarities to signaling adaptors hint at potential roles in intracellular pathways.
C5orf4 expression has been sporadically documented in tissues like brain, liver, and testes, but conflicting data exist regarding its abundance and isoform diversity. Limited studies associate it with cell cycle regulation, apoptosis modulation, and possible involvement in inflammatory responses. Intriguingly, altered C5orf4 mRNA levels have been observed in cancers (e.g., gliomas, colorectal carcinomas) and metabolic disorders, though causal relationships are unverified.
Recombinant C5orf4 is primarily used to generate antibodies or study biochemical properties, as native protein detection remains challenging due to low endogenous expression. Current research gaps include unclear interactome profiles, regulatory mechanisms, and disease relevance. Recent CRISPR screening data tentatively link C5orf4 to ER stress pathways, warranting deeper mechanistic exploration. Its uncharacterized status underscores the need for structural studies, conditional knockout models, and multi-omics profiling to clarify its role in human physiology and pathology.
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