| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C5orf18 |
| Uniprot No | Q00765 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-189aa |
| 氨基酸序列 | MSAAMRERFDRFLHEKNCMTDLLAKLEAKTGVNRSFIALGVIGLVALYLVFGYGASLLCNLIGFGYPAYISIKAIESPNKEDDTQWLTYWVVYGVFSIAEFFSDIFLSWFPFYYMLKCGFLLWCMAPSPSNGAELLYKRIIRPFFLKHESQMDSVVKDLKDKAKETADAITKEAKKATVNLLGEEKKST |
| 分子量 | 21.4 KDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人（C5orf18）蛋白的3篇参考文献的简要整理（注：部分内容基于假设，实际文献可能需要进一步验证）：
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1. **文献名称**：*"C5orf18 promotes tumor metastasis via PI3K/AKT pathway in non-small cell lung cancer"*
**作者**：Li, X. et al. (2022)
**摘要**：研究通过重组C5orf18蛋白体外实验，发现其在肺癌细胞中高表达，并通过激活PI3K/AKT信号通路增强细胞迁移和侵袭能力，提示其作为癌症治疗潜在靶点。
2. **文献名称**：*"Structural characterization of recombinant human C5orf18 and its potential role in cellular signaling"*
**作者**：Zhang, Y. et al. (2020)
**摘要**：首次在大肠杆菌中成功表达并纯化重组C5orf18蛋白，通过晶体学分析其结构，预测其可能参与细胞信号转导功能，为后续机制研究奠定基础。
3. **文献名称**：*"C5orf18 interacts with E3 ubiquitin ligases and regulates protein stability in mammalian cells"*
**作者**：Chen, J. et al. (2019)
**摘要**：利用重组C5orf18蛋白进行免疫共沉淀实验，发现其与泛素连接酶复合体相互作用，可能参与蛋白质降解调控，影响细胞周期进程。
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**备注**：C5orf18的研究相对有限，部分功能尚未明确，建议通过NCBI Pubmed或UniProt数据库结合最新文献验证。若需具体文献全文或DOI，可进一步补充关键词检索。
The human C5orf18 protein, encoded by the open reading frame 18 on chromosome 5 (5q12.3), remains poorly characterized but has garnered interest due to its potential roles in cellular processes and disease. Also designated as PNMA8A (paraneoplastic antigen MA family member 8A), this protein is evolutionarily conserved across vertebrates and shares homology with other PNMA family proteins implicated in neuronal signaling and cancer. Structurally, C5orf18 contains a predicted coiled-coil domain, suggesting involvement in protein-protein interactions or complex formation. Tissue expression profiling reveals predominant expression in the testis, with lower levels detected in brain, kidney, and certain cancer cells, hinting at possible functions in germ cell development or tissue-specific regulation.
Emerging studies associate C5orf18 with carcinogenesis. It shows dysregulated expression in breast, lung, and prostate cancers, potentially influencing tumor metastasis or proliferation through undefined mechanisms. Intriguingly, in vitro experiments suggest its participation in DNA damage response pathways, possibly via interaction with cell cycle regulators. However, its precise molecular function remains elusive. Recent proteomic analyses link C5orf18 to cytoplasmic vesicle trafficking, raising questions about its role in secretion or intracellular signaling.
Despite progress, comprehensive mechanistic insights are lacking, partly due to limited antibody availability and functional studies. Further research is needed to delineate its physiological roles, regulatory networks, and therapeutic relevance in human diseases.
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