| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C4orf6 |
| Uniprot No | Q99440 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-93aa |
| 氨基酸序列 | MDTQKQIHKT HNSKNQFFTI FFFLSVEFGK EGTRKNFYLL LSIGHYGRKS RRADLGTADT ADKTEPECFA ASWTFDPNPS VTVSGAHSTA VHQ |
| 分子量 | 10.4 KDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
由于目前关于C4orf6的研究较为有限，且该蛋白的功能尚未完全阐明，以下为**模拟示例**的参考文献，供参考：
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1. **文献名称**：*"Cloning and Expression of Recombinant Human C4orf6: A Potential Biomarker in Colorectal Cancer"*
**作者**：Wang et al.
**摘要**：报道了C4orf6的基因克隆及在大肠杆菌中的重组表达，并通过免疫组化发现其在结直肠癌组织中表达上调，提示其可能与肿瘤进展相关。
2. **文献名称**：*"Bioinformatic Analysis and Subcellular Localization of C4orf6 Protein"*
**作者**：Gupta & Lee
**摘要**：利用生物信息学工具预测C4orf6含有跨膜结构域，并通过荧光标记实验证实其定位于细胞质中的内质网，推测其参与分泌途径调控。
3. **文献名称**：*"C4orf6 Interacts with HSP70: Implications for Cellular Stress Response"*
**作者**：Chen et al.
**摘要**：通过免疫共沉淀技术发现C4orf6与热休克蛋白HSP70存在物理相互作用，可能通过调控应激反应影响细胞存活。
4. **文献名称**：*"Silencing C4orf6 Inhibits Migration in Breast Cancer Cells"*
**作者**：Rodriguez et al.
**摘要**：利用siRNA敲降C4orf6后，乳腺癌细胞的迁移能力显著下降，提示其可能在肿瘤转移中发挥作用。
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**注**：以上文献为假设性示例，实际研究中需通过**PubMed、Google Scholar或UniProt数据库**检索真实文献。若C4orf6的研究较新，建议关注预印本平台（如bioRxiv）或高通量组学数据的次级分析。
C4orf6 (Chromosome 4 Open Reading Frame 6), also designated as FLJ20581 or HSPC126, is a poorly characterized human protein encoded by a gene located on chromosome 4p16.1. Despite its identification over two decades ago, limited experimental data exist regarding its structure, function, or physiological relevance. Bioinformatics analyses suggest it encodes a small cytoplasmic/nuclear protein (~20 kDa) with intrinsically disordered regions, potential phosphorylation sites, and a conserved motif of unknown significance across vertebrates. While its molecular interactions remain unvalidated, weak associations with RNA-binding proteins and components of the ubiquitin-proteasome system have been proposed via high-throughput studies. Recombinant C4orf6 is occasionally used in antibody validation or interaction screens, though reproducibility issues persist due to undocumented splice variants and conflicting subcellular localization reports. Some transcriptomic datasets link altered C4orf6 expression to developmental disorders and cancers, but causal relationships remain unproven. Current challenges in studying this protein include the absence of knockout models, ambiguous functional domains, and insufficient clinical correlations. Recent interest in understudied "dark" proteomes has prompted renewed exploration of C4orf6 through CRISPR-based functional genomics and structural prediction tools like AlphaFold, though experimental validation remains critical. (Word count: 199)
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