| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C4orf41 |
| Uniprot No | Q7Z392 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-739aa |
| 氨基酸序列 | MSCVSSYAGFDLSDPEKEKVGILAIQLKERNVVHSEIIITLLSNAVAQFKKYKCPRMKSHLMVQMGEEYYYAKDYTKALKLLDYVMCDYRSEGWWTLLTSVLTTALKCSYLMAQLKDYITYSLELLGRASTLKDDQKSRIEKNLINVLMNESPDPEPDCDILAVKTAQKLWADRISLAGSNIFTIGVQDFVPFVQCKAKFHAPSFHVDVPVQFDIYLKADCPHPIRFSKLCVSFNNQEYNQFCVIEEASKANEVLENLTQGKMCLLPGKTRKLLFKFVAKTEDVGKKIEITSVDLALGNETGRCVVLNWQGGGGDAASSQEALQAARSFKRRPKLPDNEVHWDSIIIQASTMIISRVPNISVHLLHEPPALTNEMYCLVVTVQSHEKTQIRDVKLTAGLKPGQDANLTQKTHVTLHGTELCDESYPALLTDIPVGDLHPGEQLEKMLYVRCGTVGSRMFLVYVSYLINTTVEEKEIVCKCHKDETVTIETVFPFDVAVKFVSTKFEHLERVYADIPFLLMTDLLSASPWALTIVSSELQLAPSMTTVDQLESQVDNVILQTGESASECFCLQCPSLGNIEGGVATGHYIISWKRTSAMENIPIITTVITLPHVIVENIPLHVNADLPSFGRVRESLPVKYHLQNKTDLVQDVEISVEPSDAFMFSGLKQIRLRILPGTEQEMLYNFYPLMAGYQQLPSLNINLLRFPNFTNQLLRRFIPTSIFVKPQGRLMDDTSIAAA |
| 分子量 | 109.4 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C4orf41蛋白的**示例性参考文献**（基于假设性研究内容，建议通过PubMed或专业数据库核实真实文献）：
1. **文献名称**：*"Functional characterization of C4orf41 as a regulator of mitochondrial dynamics"*
**作者**：Chen L, et al.
**摘要**：本研究通过重组表达人C4orf41蛋白，发现其与线粒体分裂蛋白DRP1相互作用，调控细胞能量代谢与凋亡，提示其在神经退行性疾病中的潜在作用。
2. **文献名称**：*"Structural insights into the catalytic domain of human C4orf41 protein"*
**作者**：Kim J, et al.
**摘要**：通过X射线晶体学解析重组C4orf41蛋白的三维结构，揭示其具有类水解酶活性位点，为探索其在DNA修复中的分子机制提供依据。
3. **文献名称**：*"C4orf41 knockdown promotes tumor progression via Wnt/β-catenin signaling"*
**作者**：Wang X, et al.
**摘要**：利用CRISPR技术敲低C4orf41基因，并表达其重组蛋白进行回补实验，证明其通过抑制Wnt通路抑制结直肠癌细胞迁移。
4. **文献名称**：*"Recombinant C4orf41 protein alleviates inflammation in sepsis models"*
**作者**：Gupta R, et al.
**摘要**：在大肠杆菌中重组表达并纯化C4orf41蛋白，发现其通过调节NF-κB通路减少巨噬细胞炎症因子释放，为脓毒症治疗提供新靶点。
**注意**：C4orf41尚属研究较少的基因，上述内容为假设性示例。建议使用[NCBI Gene](https://www.ncbi.nlm.nih.gov/gene)或[UniProt](https://www.uniprot.org)查询最新功能注释，并通过关键词“C4orf41 recombinant protein”在PubMed检索真实文献。
**Background of Recombinant Human C4orf41 Protein**
The C4orf41 (Chromosome 4 Open Reading Frame 41) protein, also referred to as CRPPA (Chromosome 4 Reading Frame Protein A), is a poorly characterized protein encoded by the *C4orf41* gene located on human chromosome 4 (4q21.21). While its precise molecular function remains unclear, bioinformatic analyses suggest potential roles in nucleic acid metabolism or protein-protein interactions due to conserved domains and structural homology with other RNA/DNA-binding proteins.
Expression of *C4orf41* is broadly detected in tissues such as the brain, testis, and liver, hinting at tissue-specific functions. Studies link it to cellular stress responses and cancer progression; for instance, its dysregulation has been observed in hepatocellular carcinoma and glioblastoma. Recombinant human C4orf41 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), enables functional studies, including interactome mapping and enzymatic assays. However, experimental validation of its biological roles is limited, with most data derived from high-throughput screens or *in silico* predictions.
Recent interest in C4orf41 stems from its potential involvement in neurological disorders and its interaction with proteins associated with DNA repair pathways. Despite these findings, comprehensive mechanistic insights are lacking. Further research is necessary to clarify its physiological significance, disease associations, and therapeutic potential. Recombinant C4orf41 serves as a critical tool for such investigations, bridging gaps between genomic data and functional annotation.
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