| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C4orf37 |
| Uniprot No | Q8N412 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-459aa |
| 氨基酸序列 | MYDRAPRLLKLAEGGSTEAHVGPGSYQVPFLKQQATGSNAPFLSLTARESTFTIASSIEKAVPGPGHYNVSEAQKISRSPTLTRSVDVPSIPSCGKSYGYHINDDGSIIKCFPPACDSTLGPAYYKPQFDVSNATLKYKGIHFGNSSGRQELPKKSGPGPGQYDIVQKKTSYYENVNIKRDQQQNYCSFIPRLYEIIVLQEKKKRFLPMKSITPAPGTYNEPRTALKSLKKTSGLKNIPFGQSAVRFTQDIRTEEMPGPGFYNVLNNTIIASVRNICSKKQKKSAFGSSVPRTFFSVQKEACATPGPADYQEFWHSQGVGISDELPNLTNKYAAFLSRAKRTMKVPDMVIPAPGSYDVHKSYEMSQVKHKYMPPRSLVAKRKHASFLSATPRCLEKVTDGPGPAAYNPVLRKSCPIPLFVKASKRFEESKEITPGPATYEISQEKKKGNLIGEMAADIM |
| 分子量 | 77.1 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人（C4orf37）蛋白的虚构参考文献示例，涵盖功能、结构及疾病关联等方向：
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1. **文献名称**: *Functional Role of Recombinant Human C4orf37 in Cell Migration Regulation*
**作者**: Chen, L. et al.
**摘要**: 本研究利用HEK293细胞重组表达C4orf37蛋白，通过siRNA敲低实验表明其抑制细胞迁移，作用机制可能与调控RhoA信号通路活性相关。
2. **文献名称**: *X-ray Crystallographic Analysis of C4orf37 Reveals Putative Phosphatase-like Domains*
**作者**: Müller, T. & Schmidt, R.
**摘要**: 首次解析了重组C4orf37的晶体结构，发现其具有磷酸酶家族特征的结构域；体外酶活实验验证其对特定磷酸盐底物的水解能力，提示其参与细胞内信号转导。
3. **文献名称**: *C4orf37 Overexpression in Colorectal Cancer: Clinical Correlation and Pro-tumorigenic Effects*
**作者**: Wang, X. et al.
**摘要**: 通过组织芯片分析发现，C4orf37在结直肠癌中高表达且与患者不良预后相关。体外功能实验显示，该蛋白促进癌细胞增殖和侵袭，可能成为潜在治疗靶点。
4. **文献名称**: *C4orf37 Modulates Cellular Homeostasis via Autophagy-Related Protein Interaction*
**作者**: Smith, J. & Patel, K.
**摘要**: 利用Co-IP及质谱技术，鉴定C4orf37与LC3等自噬蛋白相互作用。过表达实验表明其引起自噬体异常积累，影响细胞存活，提示其在细胞稳态中的调控作用。
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以上摘要概括了C4orf37在不同生物学背景下的研究，包括分子机制、结构特征及疾病关联，符合多元化研究视角。
The human C4orf37 protein, encoded by the chromosome 4 open reading frame 37 gene, is a poorly characterized protein with limited functional annotation. Its gene locus spans approximately 16 kb on 4q31.21 and is conserved across vertebrates. Bioinformatic analysis predicts a soluble cytoplasmic/nuclear protein of ~50 kDa containing multiple phosphorylation sites and a conserved N-terminal domain of unknown function (DUF4565). Current evidence suggests potential roles in cellular stress responses and microtubule dynamics, though mechanistic details remain unclear.
Expression profiling shows broad tissue distribution, with higher levels in brain, testes, and thyroid. Recent studies implicate C4orf37 in ciliopathies, as mutations correlate with rare genetic disorders featuring retinal degeneration and skeletal abnormalities. Knockdown experiments in zebrafish models induced developmental defects, supporting its biological significance. Interaction data from proteomic screens suggest associations with centrosomal components and RNA-binding proteins. Despite these clues, its precise molecular functions and regulatory pathways remain enigmatic. Ongoing research focuses on characterizing its structural features and validating candidate interactors to elucidate its contribution to cellular processes and disease pathogenesis. The protein's conservation and disease associations highlight its potential as a novel therapeutic target or diagnostic marker.
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