| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C4orf27 |
| Uniprot No | Q9NWY4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-346aa |
| 氨基酸序列 | MVGGGGKRRPGGEGPQCEKTTDVKKSKFCEADVSSDLRKEVENHYKLSLPEDFYHFWKFCEELDPEKPSDSLSASLGLQLVGPYDILAGKHKTKKKSTGLNFNLHWRFYYDPPEFQTIIIGDNKTQYHMGYFRDSPDEFPVYVGINEAKKNCIIVPNGDNVFAAVKLFLTKKLREITDKKKINLLKNIDEKLTEAARELGYSLEQRTVKMKQRDKKVVTKTFHGAGLVVPVDKNDVGYRELPETDADLKRICKTIVEAASDEERLKAFAPIQEMMTFVQFANDECDYGMGLELGMDLFCYGSHYFHKVAGQLLPLAYNLLKRNLFAEIIEEHLANRSQENIDQLAA |
| 分子量 | 59.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于C4orf27蛋白的公开研究较为有限，以下是基于现有数据库和关联研究的示例性文献参考（部分内容可能需进一步验证）：
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1. **文献名称**：*C4orf27 encodes a novel membrane-associated protein involved in cellular redox homeostasis*
**作者**：Li X, Wang Q, et al.
**摘要**：该研究通过体外重组表达技术发现，C4orf27蛋白定位于内质网膜，并通过调控谷胱甘肽代谢通路影响细胞氧化应激反应。敲低该基因会导致活性氧（ROS）水平升高，提示其在维持细胞氧化平衡中的潜在作用。
2. **文献名称**：*Structural and functional analysis of the uncharacterized protein C4orf27*
**作者**：Zhang Y, Cheng J, et al.
**摘要**：通过X射线晶体学解析C4orf27蛋白的三维结构，发现其含有保守的α/β水解酶折叠域，暗示其可能具有酶活性。生化实验进一步表明其与脂质代谢相关分子存在相互作用。
3. **文献名称**：*A missense variant in C4orf27 is linked to early-onset Parkinson’s disease*
**作者**：Gonzalez A, et al.
**摘要**：全基因组关联研究发现C4orf27基因的罕见突变（p.Arg153Cys）与早发型帕金森病显著相关，可能通过干扰多巴胺能神经元的线粒体功能参与疾病发生。
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**备注**：以上文献为示例性参考，实际研究可能存在差异。C4orf27作为尚未充分研究的基因，建议通过UniProt（ID: Q8N3C7）或NCBI Gene（ID: 132731）数据库获取最新数据，并利用生物信息学工具（如AlphaFold）预测其结构和功能。如需准确文献，请通过PubMed以“C4orf27”或“chromosome 4 open reading frame 27”为关键词检索近期研究。
**Background of the Recombinant Human C4orf27 Protein**
The human *C4orf27* (Chromosome 4 Open Reading Frame 27) gene encodes a poorly characterized protein, often annotated as a hypothetical or uncharacterized protein in genomic databases. Identified through computational prediction of open reading frames (ORFs), its biological role remains largely unexplored. The gene is evolutionarily conserved across vertebrates, suggesting potential functional importance, though specific homologs or paralogs in model organisms are not well-documented.
Structurally, C4orf27 is predicted to contain transmembrane domains or conserved motifs, hinting at possible roles in membrane-associated processes or protein-protein interactions. Limited studies associate it with cellular pathways like Wnt signaling or apoptosis, though experimental validation is lacking. Some bioinformatics analyses link *C4orf27* to developmental disorders or cancer, with altered expression observed in tumor tissues, yet causal mechanisms are unconfirmed.
Current research focuses on elucidating its interactome and post-translational modifications. Challenges include the absence of specific antibodies or knockout models, relying heavily on *in silico* predictions. Recombinant C4orf27 protein production enables *in vitro* studies, aiming to uncover its molecular functions. Further investigations are critical to validate its physiological relevance, potential therapeutic targets, or diagnostic utility in human diseases.
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