关键信息
-
基因名
SGK1
- 应用
-
别名
SGK1;SGK;Serine/threonine-Protein kinase Sgk1
-
种属
Human
-
表达系统
E. coli
-
标签
His tag N-Terminus
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
O00141
-
表达区间
1-431aa
-
氨基酸序列
MTVKTEAAKG TLTYSRMRGM VAILIAFMKQ RRMGLNDFIQ KIANNSYACK HPEVQSILKI SQPQEPELMN ANPSPPPSPS QQINLGPSSN PHAKPSDFHF LKVIGKGSFG KVLLARHKAE EVFYAVKVLQ KKAILKKKEE KHIMSERNVL LKNVKHPFLV GLHFSFQTAD KLYFVLDYIN GGELFYHLQR ERCFLEPRAR FYAAEIASAL GYLHSLNIVY RDLKPENILL DSQGHIVLTD FGLCKENIEH NSTTSTFCGT PEYLAPEVLH KQPYDRTVDW WCLGAVLYEM LYGLPPFYSR NTAEMYDNIL NKPLQLKPNI TNSARHLLEG LLQKDRTKRL GAKDDFMEIK SHVFFSLINW DDLINKKITP PFNPNVSGPN DLRHFDPEFT EEPVPNSIGK SPDSVLVTAS VKEAAEAFLG FSYAPPTDSF L
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
SGK1 (serum/glucocorticoid-regulated kinase 1) is a serine/threonine kinase that plays a critical role in various cellular processes, including cell survival, proliferation, and metabolism. Its relevance is highlighted by its involvement in signaling pathways related to stress response and hormonal regulation, particularly in response to insulin and glucocorticoids. Dysregulation of SGK1 has been associated with several pathological conditions, including cancer, metabolic disorders, and cardiovascular diseases, making it a promising target for therapeutic intervention. Research on recombinant SGK1 protein has gained significance as it allows for a better understanding of the enzyme's structure-function relationship, catalytic mechanisms, and interaction with downstream substrates. Notably, the production of recombinant SGK1 in suitable expression systems enables the detailed study of its activity and regulation in vitro, facilitating the exploration of its potential as a drug target. Additionally, understanding SGK1's role in disease mechanisms can lead to novel strategies for treatment and provide insights into how manipulation of its activity may influence cellular outcomes. Consequently, the study of SGK1 recombinant protein continues to be a focal point in biomedical research, striving to unravel its complex biological functions and therapeutic potential.
