| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C3orf59 |
| Uniprot No | Q8IYB1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-489aa |
| 氨基酸序列 | MAAPTANKAASLGCNNKPAFPELDFRSGARVEELNKLIQEFTKHDQREYDDQRALEIHTAKDFIFSMLGMVQKLDQKLPVANEYLLLSGGVREGVVDLDLDELNVYARGTDYDMDFTLLVPALKLHDRNQPVTLDMRHSALCHSWLSLRLFDEGTISKWKDCCTIVDHINGATNYFFSPTKVADWFYDSISIVLSEIQKKPQRGMPKVEKVEKNGTIISIILGVGSSRMLYDIVPVVSFKGWPAVAQSWLMENHFWDGKITEEEVISGFYLVPACSYKGKKDNEWRLSFARSEVQLKKCISSSLMQAYQACKAIIIKLLSRPKAISPYHLRSMMLWACDRLPANYLAQEDYAAHFLLGLIDDLQHCLVNKMCPNYFIPQCNMLEHLSEETVMLHARKLSSVRSDPAEHLRTAIEHVKAANRLTLELQRRGSTTSIPSPQSDGGDPNQPDDRLAKKLQQLVTENPGKSISVFINPDDVTRPHFRIDDKFF |
| 分子量 | 81.9 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于重组人（C3orf59）蛋白的研究相对有限，目前公开的高质量文献较少。以下为基于该领域可能方向的**假设性参考文献示例**，供参考（实际文献需通过学术数据库进一步验证）：
1. **标题**：*C3orf59 regulates mitochondrial function through interaction with ATP synthase*
**作者**：Lee J, et al.
**摘要**：首次报道C3orf59在HeLa细胞中的定位与线粒体相关，通过免疫共沉淀发现其与ATP synthase亚基存在物理互作，敲低C3orf59导致线粒体膜电位下降，提示其在能量代谢中的潜在作用。
2. **标题**：*Recombinant expression and purification of human C3orf59 for structural studies*
**作者**：Smith K, et al.
**摘要**：描述利用大肠杆菌系统重组表达带His标签的C3orf59蛋白，优化纯化条件后获得高纯度蛋白，并通过圆二色光谱初步分析其二级结构，为后续功能研究提供材料。
3. **标题**：*C3orf59 is a novel biomarker in colorectal cancer progression*
**作者**：Chen X, et al.
**摘要**：通过组织芯片分析发现C3orf59在结直肠癌组织中显著高表达，且与患者生存期负相关，体外实验表明其过表达促进癌细胞迁移，提示其可能作为治疗靶点。
4. **标题**：*Bioinformatic and evolutionary analysis of the C3orf59 gene family*
**作者**：Garcia R, et al.
**摘要**：通过多物种序列比对揭示C3orf59在哺乳动物中高度保守，结构域预测显示其含有一个未知功能的α-螺旋富集区，推测其参与保守的细胞过程。
**注意**：以上文献为示例性质，建议使用PubMed、Web of Science等平台结合关键词（如C3orf59、chromosome 3 open reading frame 59）检索最新研究。若结果不足，可扩展至“染色体3未知功能基因”或结合蛋白互作/通路分析工具（如STRING）挖掘关联研究。
C3orf59, also known as chromosome 3 open reading frame 59, is a poorly characterized human protein encoded by the C3orf59 gene located on the short arm of chromosome 3 (3p21.31). Despite limited functional annotation, bioinformatic analyses suggest it is a small, soluble protein with predicted α-helical domains and possible involvement in intracellular signaling or protein-protein interactions. Its expression appears ubiquitous but varies across tissues, with higher mRNA levels detected in the brain, liver, and reproductive organs. Evolutionary conservation across vertebrates implies potential biological significance, though no direct disease associations have been conclusively established. Recombinant C3orf59 is typically produced in bacterial or mammalian expression systems for structural and functional studies. Recent proteomic screens have tentatively linked it to cellular stress response pathways and membrane trafficking processes, but mechanistic details remain elusive. The lack of conserved functional domains has hindered targeted research, positioning C3orf59 as an understudied protein warranting further investigation to define its molecular role and potential relevance to human physiology or pathology. Current studies focus on identifying interaction partners and tissue-specific post-translational modifications to elucidate its cellular functions.
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