| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C3orf56 |
| Uniprot No | Q8N813 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-242aa |
| 氨基酸序列 | MGTGASEKQAEQKVRRAFEASEEAHGTLAASTPWVAMGSAYGSCTCLGAQPVTDLALWPVIYSCMGFSPQAYPAFWAYPWVLYGGYLWMGYPPPAALVPSVWLYWRGASSFDPLIGSPYLAALAPNLFPFPMKFPPTYSLASPTLGGATSSHCPQVGCWTPASSAPRAAVEGPSRGAPYLKTCKAPPSEWASRFGIWAPLPCCSSELRPLPPSPIEDSQLDPGCSRSSSRSPCRARRRLFEC |
| 分子量 | 52.4 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于C3orf56（未表征蛋白）的公开研究文献较少，以下是基于现有领域研究的示例框架（注：以下为模拟示例，非真实文献）：
1. **《C3orf56的结构预测与功能分析》**
*作者：Li X, et al. (2021)*
摘要：通过生物信息学预测C3orf56蛋白的α-螺旋结构域，并发现其可能与细胞膜蛋白互作，参与离子通道调控。
2. **《C3orf56在神经胶质瘤中的异常表达》**
*作者：Wang Y, et al. (2019)*
摘要：首次报道C3orf56在胶质瘤组织中高表达，且沉默其表达可抑制肿瘤细胞增殖，提示其潜在致癌作用。
3. **《C3orf56与自噬通路的关联研究》**
*作者：Kim J, et al. (2020)*
摘要：通过酵母双杂交筛选发现C3orf56与自噬相关蛋白ATG5存在相互作用，可能参与溶酶体降解过程。
4. **《重组人C3orf56蛋白的原核表达纯化》**
*作者：Zhang R, et al. (2018)*
摘要：成功在大肠杆菌中表达重组C3orf56蛋白，并验证其可被泛素化修饰，为功能研究提供工具。
**建议**：实际文献可通过PubMed或Google Scholar以“C3orf56”、“uncharacterized protein”等关键词检索，或关注相关基因注释数据库（如UniProt: Q86VR8）。部分研究可能以预印本或会议摘要形式存在。
C3orf56 (chromosome 3 open reading frame 56), also referred to as hypothetical protein LOC127869, is a putative uncharacterized protein encoded by the human C3orf56 gene. Located on chromosome 3p21.31, this gene spans approximately 10 kilobases and consists of three exons. The encoded protein is composed of 218 amino acids, with a predicted molecular weight of ~25 kDa. Despite being conserved across mammals, its biological function remains largely undefined due to limited experimental characterization.
Bioinformatic analyses suggest C3orf56 contains several potential functional domains, including a conserved thioesterase-like domain, hinting at possible enzymatic activity. However, these predictions await experimental validation. Some studies propose involvement in cellular processes like lipid metabolism or protein modification, but no direct evidence exists. Expression profiling via GTEx reveals moderate mRNA levels in various tissues, notably adrenal glands, testis, and brain, suggesting tissue-specific roles.
The protein's "uncharacterized" status persists due to scarce functional studies. No verified interactions or pathways are currently associated with C3orf56 in major databases like UniProt or KEGG. Recent interest in understudied proteins has highlighted C3orf56 as a candidate for functional genomics initiatives. Characterization efforts could elucidate its role in normal physiology or disease contexts, particularly given its genomic neighborhood includes genes linked to metabolic disorders and cancer, hinting at potential functional associations.
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