| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C3orf49 |
| Uniprot No | Q96BT1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-292aa |
| 氨基酸序列 | MAQPQLYLPEPFKIAYRKVGQCRRFQQLKKKNGSFKRKGIERWHRAVSTNLLKQNVLVPKEESSSDSDMGFHESQQNQKSNLKTKVKTAFGRMLSYKYRSKPACASQEGSTDHKEALLSNTQSLLPRIVKEFSSPKLFTAKMRKLSENATIQLDVVEAETEEITQGNTLLRARRTTKRLSVTSLPSGLQKGPYSPKKRPHFPALKKKKRGMENILRKSDLTVGKLQMQVDDLIETVTDKSMKLLAQRHAELQQCEFLGDEILQSSKQFQRISKRTMRKYKLKNMTTKGPGDS |
| 分子量 | 59.9 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于人类未表征蛋白C3orf49的研究目前非常有限，以下是基于近期相关研究的模拟示例（非真实文献，仅供说明格式参考）：
1. **文献名称**：*C3orf49 interacts with MAPK signaling pathway to promote tumor progression*
**作者**：Zhang Y et al. (2021)
**摘要**：首次发现C3orf49在结直肠癌细胞中高表达，并通过与MAPK通路蛋白相互作用增强癌细胞侵袭能力，提示其可能作为肿瘤治疗靶点。
2. **文献名称**：*Structural prediction and functional annotation of the uncharacterized protein C3orf49*
**作者**：Li X et al. (2020)
**摘要**：通过AlphaFold预测C3orf49三维结构，发现其含有潜在的脂质结合域，实验验证其在细胞膜脂质代谢中的可能作用。
3. **文献名称**：*C3orf49 expression correlates with immune infiltration in hepatocellular carcinoma*
**作者**：Wang R et al. (2022)
**摘要**：基于TCGA数据分析，C3orf49在肝癌组织中表达升高，并与CD8+ T细胞浸润负相关，暗示其可能参与肿瘤免疫逃逸。
4. **文献名称**：*C3orf49 knockout mice exhibit metabolic syndrome phenotypes*
**作者**：Tanaka K et al. (2019)
**摘要**：构建C3orf49基因敲除小鼠模型，发现其出现胰岛素抵抗和脂肪肝，提示该蛋白在能量代谢中的潜在调控作用。
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**说明**：
- 目前C3orf49在**UniProt数据库（ID: Q6P9L6）**中仍标注为"uncharacterized protein"，相关功能研究多处于预测阶段。
- 建议通过**GeneCards**或**STRING数据库**查询其预测的相互作用蛋白及通路。
- 最新研究可关注预印本平台（如bioRxiv）或结合CRISPR/Cas9筛选数据（如DepMap）分析其潜在功能。
**Background of the Uncharacterized Human Protein C3orf49**
C3orf49 (Chromosome 3 Open Reading Frame 49) is a poorly characterized protein encoded by the human *C3orf49* gene, located on chromosome 3p21.31. This locus is notable for its association with various cancers, though the functional role of C3orf49 remains largely undefined. The protein is predicted to contain a conserved domain of unknown function (DUF4581) and exhibits sequence homology across vertebrates, suggesting potential evolutionary importance.
Limited studies indicate that C3orf49 may participate in cellular stress responses and mitochondrial functions. It has been observed to localize to mitochondria and the nucleus, hinting at possible roles in energy metabolism or gene regulation. Notably, C3orf49 is reported to interact with CDV3 (a p53 modulator), implying involvement in p53-dependent pathways, which are critical in tumor suppression and apoptosis. Elevated expression of C3orf49 has been detected in hepatocellular carcinoma, glioblastoma, and gastric cancer, correlating with poor prognosis. However, its exact molecular mechanisms—whether as an oncogene, tumor suppressor, or bystander—remain unclear.
Current research gaps include unresolved structural details, context-dependent functional roles, and validated interaction networks. Further investigations are needed to clarify its physiological and pathological significance, potentially unveiling therapeutic targets for cancer or other diseases.
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