| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C3orf44 |
| Uniprot No | Q7L0X2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-517aa |
| 氨基酸序列 | MSEMSIDRNIHRNLSPGIPVSVQTEESWLQDLSDKVQSRKKASKEKAEPECLASKLREKWVINPEESKLNILYELEFKEDFITLFEPSLRTLPSIGPPSILAYKEESSNLGINFKDEEEETSPKCEFCGSDLRAFFSNVDVSSEPKGHASCCIAFQNLIDYIYEEQIKTKPPKAELIAIDPHAAHGSEVDRLKAKEKALQRKQEQRMARHFAIISREQTHFSEDDSKRLKTISYQLSVDIPEKQIIDDIVFDFQLRNSNMSIICCDSRIACGKVVRNELLEKHYKHGSKFLTSFPDGTTQIFYPSGNLAIIRVPNKVNGFTCIVQEDMPTNPAILAVLDSSGRSSCYHPNGNVWVYINILGGQYSDQAGNRIRAWNWSNSITSSPFVSFKPVFLALNRYIGVRILEQDKISITFLAMGQQARISVGTKVKLPNPEEIPILRYVSGDDLLLLASLIKIRRLFHKLEGCVNFPSSQVWEKLKQPSYLSSLSLKLIALCHSSGIKQDIMKTIRNIINEEI |
| 分子量 | 85 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C3orf44蛋白的3篇示例参考文献，内容为假设性归纳，仅供参考：
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1. **文献名称**: Recombinant Human C3orf44 Protein: Expression, Purification, and Initial Functional Characterization
**作者**: Zhang L, et al.
**摘要**: 本研究利用大肠杆菌系统成功表达并纯化重组人C3orf44蛋白。初步功能实验表明，该蛋白可能与细胞周期调控相关，并在体外实验中显示出对HeLa细胞增殖的抑制作用。
2. **文献名称**: C3orf44 Interacts with p53 and Modulates Its Transcriptional Activity
**作者**: Smith J, et al.
**摘要**: 通过免疫共沉淀和酵母双杂交实验，发现重组C3orf44蛋白与肿瘤抑制因子p53直接相互作用，可能增强p53在DNA损伤应答中的转录活性。
3. **文献名称**: Structural Insights into C3orf44 Reveal a Putative ATP-Binding Domain
**作者**: Chen H, et al.
**摘要**: 利用重组表达技术获得C3orf44蛋白，并通过X射线晶体学解析其结构。结果显示其C端存在类似ATP酶的结构域，提示其可能参与细胞能量代谢。
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**注意**：以上文献为示例，实际研究中可能需要根据具体数据库（如PubMed、Google Scholar）检索最新成果。建议使用关键词“C3orf44 recombinant protein”或“C3orf44 functional study”获取真实文献。
The human C3orf44 protein, encoded by the chromosome 3 open reading frame 44 gene, remains poorly characterized in current scientific literature. Despite its discovery through genomic sequencing, its physiological function, structural features, and regulatory mechanisms are largely undefined. Evolutionary conservation across mammalian species suggests potential biological significance, though functional studies remain sparse. Limited published data indicate possible involvement in cellular stress responses, with expression variations observed in certain cancers and neurodegenerative conditions. Hypothesized roles include potential participation in protein-protein interactions, intracellular trafficking, or DNA repair pathways based on sequence motifs and weak homology to known functional domains. Subcellular localization studies show conflicting results, with both nuclear and mitochondrial associations proposed. The protein's molecular weight (~30-35 kDa) and putative post-translational modifications (e.g., phosphorylation sites) suggest regulatory complexity. Current challenges in characterization stem from low natural abundance and lack of specific antibodies for detection. Recent transcriptomic analyses have identified differential expression patterns in metabolic disorders, prompting renewed interest in its pathophysiological relevance. Several research groups are actively working to develop knockout models and biochemical assays to elucidate its molecular mechanisms. However, the absence of conclusive functional data underscores the need for systematic investigation to validate proposed roles in cellular homeostasis and disease processes.
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