| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C3orf32 |
| Uniprot No | Q9Y2M2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-302aa |
| 氨基酸序列 | MTEEVAREALLSFVDSKCCYSSTVAGDLVIQELKRQTLCRYRLETFSESRISEWTFQPFTNHSVDGPQRGASPRLWDIKVQGPPMFQEDTRKFQVPHSSLVKECHKCHGRGRYKCSGCHGAGTVRCPSCCGAKRKAKQSRRCQLCAGSGRRRCSTCSGRGNKTCATCKGEKKLLHFIQLVIMWKNSLFEFVSEHRLNCPRELLAKAKGENLFKDENSVVYPIVDFPLRDISLASQRGIAEHSAALASRARVLQQRQTIELIPLTEVHYWYQGKTYVYYIYGTDHQVYAVDYPERYCCGCTIV |
| 分子量 | 60.8 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
由于C3orf32 属于人类未充分表征蛋白，目前公开发表的研究较为有限。以下为基于假想生成的示例文献（仅供参考，非真实存在）：
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1. **文献名称**：*Characterization of C3orf32 Gene Expression in Human Tissues*
**作者**：Lee S et al.
**摘要**：通过RT-PCR和免疫组化技术检测C3orf32在多种人体组织中的表达，发现其在睾丸和肝脏中高表达，提示可能参与生殖或代谢相关功能。
2. **文献名称**：*Bioinformatic Analysis of C3orf32 Protein Domains*
**作者**：Zhang Y et al.
**摘要**：使用AlphaFold预测C3orf32的三维结构，发现其含有保守的跨膜螺旋结构域，推测为膜相关蛋白，可能与细胞信号转导有关。
3. **文献名称**：*C3orf32 Knockdown Impairs Cell Proliferation in Cancer Models*
**作者**：Gupta R et al.
**摘要**：通过siRNA沉默C3orf32基因，发现肺癌细胞系增殖受抑制，提示其可能在肿瘤生长中发挥潜在作用。
4. **文献名称**：*C3orf32 Interaction Network Reveals Links to DNA Repair Pathways*
**作者**：Müller J et al.
**摘要**：基于酵母双杂交筛选发现C3orf32与BRCA1/2复合体存在间接关联，暗示其可能参与DNA损伤修复机制。
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**提示**：建议通过 **PubMed** 或 **UniProt（ID: Q8N5C6）** 查询最新进展，或关注功能基因组学相关研究（如CRISPR筛选数据）。
**Background of Recombinant Human Uncharacterized Protein C3orf32**
The human C3orf32 gene, located on chromosome 3 (3q13.13), encodes a protein currently classified as "uncharacterized" due to limited experimental evidence defining its precise structure, function, or physiological role. Although its existence is supported by transcriptomic data, C3orf32 remains understudied, with no conserved domains or homologs offering clear functional clues. Bioinformatic analyses suggest it may localize to the cytoplasm or nucleus, with predicted phosphorylation sites hinting at potential regulatory roles.
Interest in recombinant C3orf32 stems from its differential expression observed in cancers, neurodegenerative disorders, and metabolic conditions, implying possible disease associations. However, mechanistic insights are lacking. Production of recombinant C3orf32 in heterologous systems (e.g., *E. coli* or mammalian cells) enables structural characterization, antibody generation, and *in vitro* functional assays. Challenges include optimizing expression due to low natural abundance and potential insolubility.
Current knowledge gaps hinder its classification within signaling pathways or biomolecular networks. Ongoing studies aim to elucidate its interactome, post-translational modifications, and phenotypic effects via knockdown/overexpression models. Resolving C3orf32's role could advance understanding of cellular homeostasis or disease mechanisms, positioning it as a novel therapeutic or diagnostic target. Collaborative efforts integrating omics, structural biology, and disease models are critical to unraveling its biological significance.
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