| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C3orf27 |
| Uniprot No | O15544 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-149aa |
| 氨基酸序列 | MKEALHQIVVRCSELVSSTSLPRLSVSRLQGPPDSQPLGTLGQGGWKLLGIVGSLAPETLGGLGTEFGPCTHPLPFDMVRERERDDELRQGWLLQCPQCARTLLCHCGPFLTPPSQTSSSGFQLCSLKPSGSLVTATEPLSNFAFSYFP |
| 分子量 | 43.34 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人（C3orf27）蛋白的虚构参考文献示例，基于典型研究方向整理：
1. **文献名称**：*Structural characterization of human C3orf27 and its role in cell proliferation*
**作者**：Zhang, Y. et al.
**摘要**：本研究通过X射线晶体学解析了C3orf27的三维结构，发现其包含一个未知功能的保守结构域。体外实验表明，C3orf27的敲低会抑制HeLa细胞的增殖，提示其在细胞周期调控中起潜在作用。
2. **文献名称**：*C3orf27 interacts with the PI3K/AKT pathway to promote cancer cell survival*
**作者**：Li, W. & Chen, X.
**摘要**：作者证实C3orf27在多种癌细胞中高表达，并通过免疫共沉淀发现其与PI3K调控亚基结合。功能实验显示，C3orf27通过激活AKT信号通路抑制肿瘤细胞凋亡，可能成为癌症治疗的新靶点。
3. **文献名称**：*C3orf27 knockout mice exhibit neurodevelopmental abnormalities*
**作者**：Sato, R. et al.
**摘要**：构建C3orf27基因敲除小鼠模型，发现小鼠出现大脑皮层神经元迁移障碍和突触可塑性缺陷，提示该蛋白在神经系统发育中具有关键作用，可能与某些神经发育疾病相关。
4. **文献名称**：*Proteomic analysis of C3orf27-associated protein complexes in human cells*
**作者**：Müller, S. et al.
**摘要**：通过亲和纯化联合质谱技术，鉴定出C3orf27与RNA结合蛋白及染色质修饰因子存在相互作用，表明其可能参与转录后调控或表观遗传修饰过程，但具体机制仍需进一步研究。
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注：以上文献为示例性内容，实际研究中请通过学术数据库（如PubMed、Web of Science）检索真实发表的论文。
The human **C3orf27** protein, encoded by the *C3orf27* gene located on chromosome 3 (3p21.31), remains poorly characterized, reflecting its status as an understudied open reading frame (ORF). Initially identified through genomic sequencing, it is predicted to encode a protein of ~30 kDa, though structural and functional details are limited. Bioinformatics analyses suggest the presence of conserved regions, including potential phosphorylation sites and a disordered structure, implying roles in protein interactions or signaling. Tissue expression profiling indicates moderate expression in brain, testes, and kidneys, hinting at tissue-specific functions, though cellular localization remains unresolved.
While direct functional data are sparse, *C3orf27* has been loosely associated with ciliopathies and neurodevelopmental disorders based on genetic linkage studies. Recent multi-omics datasets propose interactions with components of the ubiquitin-proteasome system, suggesting possible involvement in protein degradation or homeostasis. Disease correlations, including cancer and rare genetic syndromes, have been observed in transcriptomic screens, though mechanistic insights are lacking.
Current research focuses on elucidating its molecular interactions and pathways using proteomics and CRISPR-based screens. Despite gaps in knowledge, C3orf27 represents a potential biomarker or therapeutic target, pending deeper investigation. Its study underscores the broader challenge of functionally annotating "dark" proteins in the human genome.
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