| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C3orf17 |
| Uniprot No | Q6NW34 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-567aa |
| 氨基酸序列 | MMAAVPPGLEPWNRVRIPKAGNRSAVTVQNPGAAIDLCIAAVIKECHLVILSLKSQTLDAETDVLCAVLYSNHNRMGRHKPHLALKQVEQCLKRLKNMNLEGSIQDLFELFSSNENQPLTTKVCVVPSQPVVELVLMKVLGACKLLLRLLDCCCKTFLLTVKHLGLQEFIILNLVMVGLVSRLWVLYKGVLKRLILLYEPLFGLLQEVARIQPMPYFKDFTFPSDITEFLGQPYFEAFKKKMPIAFAAKGINKLLNKLFLINEQSPRASEETLLGISKKAKQMKINVQNNVDLGQPVKNKRVFKEESSEFDVRAFCNQLKHKATQETSFDFKCSQSRLKTTKYSSQKVIGTPHAKSIVQRFREAESFTQLSEEIQMAVVWCRSKKLKAQAIFLGNKLLKSNRLKHLEAQGTSLPKKLECIKTSICNHLLRGSGIKTSKHHLRQRRSQNKFLRRQRKPQRKLQSTLLREIQQFSQGTRKSATDTSAKWRLSHCTVHRTDLYPNSKQLLNNGVSMPVIQTKGKMIHENLRGIHENETDSWTVMQINKNSTSGTIKETDDIDDIFALMGV |
| 分子量 | 90.9 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
由于人类未表征蛋白C3orf17的研究相对较少，以下示例结合了该领域常见研究方向和假设性文献，部分文献信息可能需要通过数据库进一步验证：
1. **文献名称**: *"C3orf17 interacts with transcriptional co-repressor complexes and modulates histone deacetylase activity"*
**作者**: Zhang et al. (2020)
**摘要**: 通过酵母双杂交筛选发现C3orf17与HDAC1/2等表观遗传调控因子相互作用，体外实验表明其过表达降低组蛋白乙酰化水平，提示可能参与染色质重塑。
2. **文献名称**: *"C3orf17 is highly expressed in testis and may regulate spermatogenesis through RNA-binding motifs"*
**作者**: Gupta & Lee (2018)
**摘要**: 生物信息学预测C3orf17含有低复杂度区域和潜在RNA结合域，组织表达谱显示其在人睾丸中特异性高表达，可能与生殖细胞发育相关。
3. **文献名称**: *"Proteomic profiling identifies C3orf17 as a potential biomarker in triple-negative breast cancer"*
**作者**: Wang et al. (2021)
**摘要**: 基于癌症质谱数据库分析发现C3orf17在三阴性乳腺癌组织中显著上调，体外敲低实验显示其促进癌细胞迁移，机制可能与Wnt通路相关。
4. **文献名称**: *"Structural prediction and recombinant expression of human C3orf17 reveals intrinsic disorder properties"*
**作者**: Müller et al. (2019)
**摘要**: 通过重组表达纯化C3orf17蛋白，结合圆二色光谱分析表明其具有内在无序性特征，提示可能通过构象变化发挥动态调控功能。
**注**: 以上文献为模拟示例，实际研究中C3orf17可能已被重新命名（如C3ORF17→新符号）或功能部分明确。建议通过UniProt（ID: Q8NAC3）或PubMed的最新检索获取真实文献。
**Background of Recombinant Human Uncharacterized Protein C3orf17**
The human protein C3orf17, encoded by the *C3orf17* gene on chromosome 3, remains largely uncharacterized, with limited functional or structural data available in scientific literature. Its name derives from its genomic locus (Chromosome 3 Open Reading Frame 17), reflecting its initial identification via computational predictions rather than experimental validation. Public databases, such as UniProt and NCBI, classify C3orf17 as a "hypothetical protein" due to the absence of direct experimental evidence for its biological role.
Bioinformatic analyses suggest it may be a soluble cytoplasmic or nuclear protein, containing putative phosphorylation sites and low-complexity regions, implying potential involvement in signaling or regulatory processes. Limited transcriptomic studies indicate variable expression across tissues, with higher levels detected in the testes and brain, hinting at possible tissue-specific functions. However, its exact molecular interactions, enzymatic activities, or pathways remain undefined.
Recombinant versions of C3orf17 have been produced for preliminary studies, often via heterologous expression in *E. coli* or mammalian systems, to enable antibody generation, structural analysis (e.g., crystallography), or functional screens. Interest in C3orf17 partially stems from genomic association studies linking its locus to diseases like cancer or neurodevelopmental disorders, though causal relationships are unproven. Current research focuses on elucidating its biochemical properties, cellular localization, and potential roles in health or disease using recombinant tools.
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