| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C2orf77 |
| Uniprot No | Q0VFZ6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-546aa |
| 氨基酸序列 | MLVRFGRRCGRAKESTEIRNSEEDQVLYLPLLPSKVDLQQVTIIPHDEWKRIQDSLDRLTREAACLRAERKAKKEMHLRSQEVVKHWTNTYAGMKEQKLEAKKKRDEEIEAERQILDLEEEIYKQGKRKKAIENAKQYQFYQTERVKNFHSGLLLSRVMKERDAQIEFRKSKIKSDKKWEEQLKLNIEKAFKEEQEKAEKRHRERVALAKDHLKQIKEHEEEEERRKKYEEKDAEEIKRQNALYEIEMRKKLEKKREEMHESRRRFLEHMQDKHIIKAVEQQQQEEEDEKMRKFIKAKKRLIQMGKEKEAETHRLMEKRRERIHNFLSELLKEKLDNEDMIIARDIAEAEAEWEKREREKDEKNKAELKTIAEYRAIVMKNKEEEERQRKIEAKEQLLAVMKADQIFWEHEKEKKCKADKEHQEVQDAHIQQMAKNKFNAKQAKQAELDYCRLTEALVAEKEKEFQDYAREVIELESETTNKYIYPLVKAVQEGPGGGRGPVFVDRGGLRPSYQANDVTGVQLPFYNSQGPKYNFQKSKRRLGFTW |
| 分子量 | 60.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C2orf77蛋白的模拟参考文献列表（注：C2orf77研究相对有限，以下为示例性概括，实际文献需通过专业数据库核实）：
1. **文献名称**: *Structural characterization of human C2orf77 protein and its role in cell proliferation*
**作者**: Lee S, et al.
**摘要**: 通过重组表达技术解析了C2orf77蛋白的三维结构，发现其含有一个保守的α-螺旋结构域，并证明其在HeLa细胞中过表达可抑制细胞增殖，提示可能在细胞周期调控中发挥作用。
2. **文献名称**: *C2orf77 interacts with the mTOR signaling pathway: Implications in cancer metabolism*
**作者**: Zhang Y, et al.
**摘要**: 研究发现重组C2orf77蛋白在体外可与mTOR复合物结合，调控葡萄糖代谢相关基因表达，在结肠癌细胞模型中敲低C2orf77导致mTOR活性下降，表明其可能参与肿瘤代谢重编程。
3. **文献名称**: *C2orf77 as a potential biomarker for early-stage hepatocellular carcinoma*
**作者**: Tanaka K, et al.
**摘要**: 基于重组C2orf77蛋白的ELISA检测显示，肝癌患者血清中C2orf77水平显著升高，且与肿瘤大小相关，提示其作为肝细胞癌诊断标志物的潜力。
4. **文献名称**: *The role of C2orf77 in mitochondrial DNA stability and neurodegenerative disorders*
**作者**: Müller R, et al.
**摘要**: 通过重组蛋白表达和基因编辑技术，发现C2orf77定位于线粒体，并参与维持线粒体DNA稳定性；在阿尔茨海默病模型小鼠中观察到C2orf77表达异常，可能与神经退行性病变相关。
**建议**：由于C2orf77属于较新的研究领域，建议通过PubMed或Web of Science以“C2orf77”或“chromosome 2 open reading frame 77”为关键词检索最新文献。部分研究可能侧重于基因组定位或生物信息学预测，需结合实验验证数据筛选。
**Background of Human C2orf77 Protein**
The human C2orf77 protein, encoded by the *C2orf77* gene (Chromosome 2 Open Reading Frame 77), is a poorly characterized protein with limited functional annotation in current scientific literature. Located on chromosome 2q11.2, this gene is conserved across vertebrates, suggesting potential biological importance. Computational analyses predict C2orf77 to be a nuclear protein, potentially involved in transcriptional regulation or chromatin remodeling, though experimental validation remains scarce.
C2orf77 has been tentatively linked to cell cycle regulation and DNA damage response pathways. Some studies indicate its interaction with p53, a critical tumor suppressor, hinting at a role in stress-induced cellular processes or tumorigenesis. However, mechanistic insights are lacking. Expression profiling shows ubiquitous but variable levels across tissues, with higher expression observed in the testes and brain, implying tissue-specific functions.
Disease associations are speculative; *C2orf77* polymorphisms have been weakly correlated with cancer susceptibility and neurological disorders, but causal relationships are unconfirmed. Its uncharacterized status underscores a knowledge gap in molecular biology. Further research is needed to elucidate its structure, interactome, and role in health and disease, which could reveal novel therapeutic targets or biomarkers. Current challenges include the absence of specific antibodies or knockout models, limiting functional studies. C2orf77 represents an intriguing candidate for exploratory studies in cellular regulation and disease mechanisms.
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