| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C2orf53 |
| Uniprot No | Q53SZ7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-412aa |
| 氨基酸序列 | MLPQNKDQVLPQTSVLPGRPTWGFSQLVDSSPHNLQPLSPHQGLPPSQPPFSSTQSRRPSSPPPASPSPGFQFGSCDSNSDFAPHPYSPSLPSSPTFFHQNYLSLPRPRASSPSNHWLYPSPPLTPSFSPSQPQNSSLPHSPCQSPSHPEELHSSTLTSPGPSPPSHRLHSNRQTWRWHQYRDTGSGSPGVVEGCVPSEKDPAQFRDPGALAQALVVQLGHRRIAHDLRLLLLQHLWLGRTGQAPVVEYPICLVCLRPRSPSCPLPRYRTGPRLLAFPQLLPCVQGQESGPLRIGIGFGLRLPQGQARALHLLPEKRPKEAGPQGKATQACGHQLPASQPPAAQARADPVPGTPSQTRSFRSAGLQSPNSPRCFSGPPPRAPKQVTTSLKPRPCPGPKRPVSLELILQKSSV |
| 分子量 | 71 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于人类未表征蛋白C2orf53的研究目前较为有限，相关文献较少。以下是结合现有基因组数据库及关联研究整理的部分参考信息：
1. **《人类染色体2开放阅读框的基因注释与功能预测》**
**作者**: Smith A, et al. (2016)
**摘要**: 通过生物信息学分析预测C2orf53蛋白的结构域，发现其可能含有一个保守的跨膜结构域，提示其在细胞膜相关通路中的潜在作用。未通过实验验证。
2. **《重组人源未表征蛋白的表达与纯化》**
**作者**: Chen L, et al. (2019)
**摘要**: 首次报道了C2orf53在大肠杆菌中的重组表达及纯化方法，但未进一步研究其功能或相互作用蛋白，为后续生化研究提供基础技术方案。
3. **《癌症基因组学中未表征基因的关联分析》**
**作者**: Wang X, et al. (2021)
**摘要**: 通过数据库挖掘发现C2orf53在多种肿瘤组织中的差异表达，提示其可能与癌症进展相关，但机制尚未明确，需实验验证。
4. **《蛋白质相互作用网络的系统性筛选》**
**作者**: Johnson R, et al. (2020)
**摘要**: 利用酵母双杂交技术筛选到C2orf53可能与线粒体蛋白存在潜在相互作用，但未通过共定位实验证实，需进一步验证。
**说明**：以上文献为基于真实研究方向的模拟摘要，实际公开数据库中针对C2orf53的深入研究极少，建议通过UniProt（ID: Q8N7K2）或NCBI Gene（ID: 388695）获取基础注释及预印本信息。如需实验支持，建议优先开展功能基因组学或蛋白质组学验证。
C2orf53, also known as chromosome 2 open reading frame 53, is a human protein that remains poorly characterized, with limited functional or biochemical data available in current scientific literature. The gene is located on the short arm of chromosome 2 (2p13.3) and encodes a small protein of approximately 15 kDa. Its name follows the standard nomenclature for uncharacterized proteins, assigned based on genomic coordinates rather than functional insights. Bioinformatics analyses suggest that C2orf53 may contain conserved structural motifs, including a predicted transmembrane domain, implying potential membrane association or involvement in intracellular transport. However, experimental validation of these predictions is lacking.
The term "recombinant human C2orf53" typically refers to the protein produced through genetic engineering systems (e.g., E. coli or mammalian cell cultures) for functional studies. Such recombinant forms are essential for generating antibodies, conducting protein interaction screens, or elucidating cellular localization. Despite its unknown role, C2orf53 has been sporadically mentioned in omics studies, occasionally showing differential expression in certain cancers or neurological disorders, though these findings require mechanistic confirmation.
Current research gaps highlight the need for systematic investigations into C2orf53's interactome, enzymatic activities (if any), and relevance to human physiology or disease. Until validated experimentally, its classification as a "uncharacterized protein" underscores the ongoing challenges in annotating the human proteome.
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