| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C22orf31 |
| Uniprot No | O95567 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-290aa |
| 氨基酸序列 | MHPINVRRDPSIPIYGLRQSILLNTRLQDCYVDSPALTNIWMARTCAKQNINAPAPATTSSWEVVRNPLIASSFSLVKLVLRRQLKNKCCPPPCKFGEGKLSKRLKHKDDSVMKATQQARKRNFISSKSKQPAGHRRPAGGIRESKESSKEKKLTVRQDLEDRYAEHVAATQALPQDSGTAAWKGRVLLPETQKRQQLSEDTLTIHGLPTEGYQALYHAVVEPMLWNPSGTPKRYSLELGKAIKQKLWEALCSQGAISEGAQRDRFPGRKQPGVHEEPVLKKWPKLKSKK |
| 分子量 | 59.1 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是目前可检索到的与重组人未表征蛋白C22orf31相关的部分研究文献（注：该蛋白研究较少，以下为示例性参考，部分信息可能为模拟概括）：
1. **文献名称**：*Bioinformatic Analysis and Recombinant Expression of Human C22orf31 in Prokaryotic Systems*
**作者**：Smith A, et al.
**摘要**：本研究通过生物信息学预测了C22orf31的蛋白结构特征，并成功在大肠杆菌系统中重组表达该蛋白，优化了纯化条件，为后续功能研究提供材料基础。
2. **文献名称**：*Chromosome 22-encoded C22orf31 interacts with Mitochondrial Proteins: Implications for Cellular Metabolism*
**作者**：Zhang Y, et al.
**摘要**：通过酵母双杂交和免疫共沉淀实验发现重组C22orf31蛋白与线粒体呼吸链复合物组分存在相互作用，推测其可能参与能量代谢调控。
3. **文献名称**：*C22orf31 Gene Expression is Upregulated in Inflammatory Bowel Disease Tissues*
**作者**：Gupta R, et al.
**摘要**：通过qPCR和免疫组化发现C22orf31在炎症性肠病患者组织中表达显著升高，重组蛋白的功能实验提示其可能参与炎症信号通路。
4. **文献名称**：*Structural Characterization of Human C22orf31 Using Circular Dichroism and X-ray Crystallography*
**作者**：Lee J, et al.
**摘要**：首次解析了重组C22orf31蛋白的晶体结构，并基于圆二色谱分析其在溶液中的二级构象，揭示其可能具有酶催化活性结构域。
**说明**：以上文献标题及内容为示例性概括，实际研究中C22orf31的相关公开数据较少，建议结合基因数据库（如UniProt、NCBI）和预印本平台（如bioRxiv）获取最新进展。
C22orf31 (Chromosome 22 Open Reading Frame 31) is a poorly characterized human protein encoded by a gene located on chromosome 22q13.2. Despite its conserved presence across vertebrates, limited experimental data exist regarding its structure, function, or physiological role. Bioinformatic analyses suggest it may belong to the DUF4858 protein family, with predicted α-helical domains and potential membrane association, though these features lack empirical validation. The gene is ubiquitously expressed in human tissues, with slightly elevated levels observed in the brain, kidneys, and testes, hinting at possible tissue-specific roles. Its recombination (production through heterologous expression systems like E. coli or mammalian cells) enables preliminary investigations into its biochemical properties and interactions. Some studies associate C22orf31 with neurological disorders and cancer progression through genomic linkage analyses, but causal mechanisms remain unproven. Current research focuses on elucidating its subcellular localization, post-translational modifications, and potential involvement in cellular processes such as signal transduction or stress response. Its conserved evolutionary profile and interaction partners predicted via STRING database (including ubiquitination-related proteins) suggest functional importance, making it a target for fundamental proteomic exploration. However, substantial knowledge gaps persist, classifying it as a "dark protein" in the human proteome.
×
