| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C22orf18 |
| Uniprot No | Q9NSP4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-180aa |
| 氨基酸序列 | MSVLRPLDKLPGLNTATILLVGTEDALLQQLADSMLKEDCASELKVHLAKSLPLPSSVNRPRIDLIVFVVNLHSKYSLQNTEESLRHVDASFFLGKVCFLATGAGRESHCSIHRHTVVKLAHTYQSPLLYCDLEVEGFRATMAQRLVRVLQICAGHVPGVSALNLLSLLRSSEGPSLEDL |
| 分子量 | 45.54 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于人类C22orf18蛋白的模拟参考文献示例（请注意，以下内容为虚构示例，实际文献需通过学术数据库检索）：
1. **文献名称**："Structural and functional analysis of the C22orf18 protein in mitochondrial sulfur metabolism"
**作者**：Lee, H. et al.
**摘要**：本研究解析了C22orf18蛋白的晶体结构，发现其作为巯基丙酮酸硫转移酶（MPST）参与线粒体硫化氢（H₂S）生成通路，并验证了其在小鼠神经元中的抗氧化应激功能。
2. **文献名称**："C22orf18 overexpression promotes tumorigenesis in colorectal cancer via Wnt/β-catenin signaling"
**作者**：Zhang, Y. et al.
**摘要**：通过组织芯片分析发现，C22orf18在结直肠癌中高表达，并证明其通过激活Wnt通路促进癌细胞增殖和迁移，提示其作为潜在治疗靶点。
3. **文献名称**："A novel role of C22orf18 in regulating autophagosome-lysosome fusion"
**作者**：Kuroda, S. et al.
**摘要**：通过CRISPR筛选发现，C22orf18通过与LC3蛋白互作调控自噬体成熟，缺失该蛋白导致神经细胞中异常蛋白聚集，可能与神经退行性疾病相关。
4. **文献名称**："C22orf18 gene variants are associated with Parkinson's disease susceptibility"
**作者**：Müller, T. et al.
**摘要**：全基因组关联分析发现，C22orf18基因的rs12345多态性与帕金森病风险显著相关，动物模型显示该蛋白缺失加重多巴胺能神经元损伤。
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**检索建议**：
实际文献可通过PubMed、Google Scholar等平台搜索关键词：
`C22orf18`、`MPST`、`sulfurtransferase`、`mitochondrial function`、`cancer`。
注意验证基因别名（如MPST即由C22orf18编码）以确保查全率。
**Background of Recombinant Human C22orf18 Protein**
The human *C22orf18* gene, located on chromosome 22 (22q13.1), encodes a protein also known as Armadillo repeat-containing helical domain-containing protein 3 (ARMH3). It belongs to the Armadillo (ARM) repeat protein family, characterized by tandem repeats of ~40 amino acid motifs that mediate protein-protein interactions. C22orf18 is evolutionarily conserved and ubiquitously expressed across tissues, with higher levels in ciliated structures like the brain, kidneys, and respiratory epithelia.
Functionally, C22orf18 is implicated in ciliogenesis and cellular signaling. Studies suggest its role in primary cilia formation, where it may interact with components of the intraflagellar transport (IFT) machinery, facilitating cargo trafficking critical for ciliary assembly and signaling. Dysregulation of C22orf18 has been linked to ciliopathies, such as Joubert syndrome, and renal or developmental disorders. Additionally, it may influence pathways like Hedgehog or Wnt signaling through its ARM domains.
Emerging research highlights its potential involvement in cancer. Altered expression has been observed in hepatocellular carcinoma and breast cancer, correlating with tumor progression. Despite these associations, the precise molecular mechanisms and full interactome of C22orf18 remain poorly characterized. Recombinant C22orf18 proteins are utilized in functional studies to decipher its biological roles and therapeutic relevance. Further research is needed to clarify its physiological and pathological contributions.
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