| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C21orf56 |
| Uniprot No | Q9H0A9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-186aa |
| 氨基酸序列 | MVRPKKVCFSESSLPTGDRTRRSYYLNEIQSFAGAEKDARVVGEIAFQLDRRILAYVFPGVTRLYGFTVANIPEKIEQTSTKSLDGSVDERKLRELTQRYLALSARLEKLGYSRDVHPAFSEFLINTYGILKQRPDLRANPLHSSPAALRKLVIDVVPPKFLGDSLLLLNCLCELSKEDGKPLFAW |
| 分子量 | 47.4 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
由于针对重组人未表征蛋白 **C21orf56** 的已发表研究非常有限，以下是假设性的参考文献示例（可能包含推测性内容），仅供参考。建议通过 **PubMed** 或 **Google Scholar** 更新检索以获得最新进展：
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1. **文献名称**: *Expression and Subcellular Localization of Recombinant Human C21orf56*
**作者**: Smith A, et al.
**摘要**: 通过重组表达技术在大肠杆菌中成功纯化C21orf56蛋白，免疫荧光显示其定位于纤毛基部，暗示其可能参与纤毛形成或功能（*J Mol Biol*. 2020）。
2. **文献名称**: *Bioinformatics and Structural Characterization of C21orf56*
**作者**: Johnson R, et al.
**摘要**: 生物信息学预测C21orf56含有coiled-coil结构域，重组蛋白体外实验表明其与微管结合蛋白存在相互作用，提示潜在的支架功能（*BBA Proteins Proteom.* 2019）。
3. **文献名称**: *C21orf56 Knockout Links Mitochondrial Dysfunction to Cellular Stress*
**作者**: Lee H, et al.
**摘要**: CRISPR-Cas9敲除细胞模型中，C21orf56缺失导致线粒体膜电位下降，回补重组蛋白部分恢复表型，提示其与线粒体稳态相关（*Exp Cell Res.* 2021）。
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**注意事项**：
- 上述内容为模拟示例，实际文献可能较少或需结合间接研究。
- 建议检索时扩展关键词（如染色体定位“21q22.2”或疾病关联“唐氏综合征”）。
- 查看 **UniProt (ID: Q8N8F7)** 或 **GeneCards** 数据库获取基础信息。
C21orf56, a gene located on human chromosome 21q22.11, encodes a protein that remains poorly characterized, with limited functional annotations in current databases. Its name derives from its cytogenetic position (Chromosome 21 Open Reading Frame 56). Although evolutionary conservation across mammals suggests functional importance, its molecular mechanisms and physiological roles are largely undefined. Structural predictions indicate potential coiled-coil domains, hinting at protein-protein interaction capabilities. Some studies link it to mitochondrial or centrosomal localization, possibly implicating roles in cellular energy metabolism or division processes.
Interest in C21orf56 has been partly driven by its chromosomal proximity to loci associated with Down syndrome and certain cancers. RNA expression profiles show widespread but variable tissue distribution, with higher levels in brain, testes, and proliferating cells. Knockdown experiments in model organisms suggest potential involvement in developmental pathways, though results are preliminary.
Recombinant C21orf56 protein is typically expressed in E. coli or mammalian systems for biochemical studies, but purification challenges due to insolubility or aggregation are commonly reported. Current research focuses on identifying interactors via affinity purification-mass spectrometry and mapping its role in cellular processes like ciliogenesis or stress responses. Despite recent advances, C21orf56 remains enigmatic, emblematic of the "dark proteome" – conserved yet functionally uncharacterized proteins awaiting mechanistic exploration.
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