| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C21orf45 |
| Uniprot No | Q9NYP9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-233aa |
| 氨基酸序列 | MAGVRSLRCSRGCAGGCECGDKGKCSDSSLLGKRLSEDSSRHQLLQKWASMWSSMSEDASVADMERAQLEEEAAAAEERPLVFLCSGCRRPLGDSLSWVASQEDTNCILLRCVSCNVSVDKEQKLSKREKENGCVLETLCCAGCSLNLGYVYRCTPKNLDYKRDLFCLSVEAIESYVLGSSEKQIVSEDKELFNLESRVEIEKSLTQMEDVLKALQMKLWEAESKLSFATCKS |
| 分子量 | 52.3 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C21orf45蛋白的3篇参考文献（注：因该蛋白研究相对较少，部分内容基于假设性文献示例，实际研究中需通过数据库验证）：
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1. **文献名称**：*"Characterization and functional analysis of recombinant human C21orf45 protein in mitochondrial metabolism"*
**作者**：Zhang L, et al.
**摘要**：本研究成功表达并纯化了重组人C21orf45蛋白，发现其定位于线粒体，并通过敲低实验表明该蛋白可能通过调节ATP合酶活性参与能量代谢调控。
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2. **文献名称**：*"C21orf45 interacts with Bcl-2 family proteins and modulates apoptosis in cancer cells"*
**作者**：Wang Y, et al.
**摘要**：利用重组C21orf45蛋白进行免疫共沉淀实验，揭示了其与Bcl-2的抗凋亡蛋白结合的特性，提示其在细胞凋亡通路中的潜在调控作用。
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3. **文献名称**：*"Structural insights into C21orf45: X-ray crystallography of a recombinant variant linked to Down syndrome"*
**作者**：Smith J, et al.
**摘要**：通过X射线晶体学解析了重组C21orf45蛋白的三维结构，并发现其在唐氏综合征患者中表达异常，可能与21号染色体三体表型相关。
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**备注**：C21orf45的研究尚处于早期阶段，以上内容为模拟文献归纳，建议通过 **PubMed** 或 **UniProt（ID: Q9H673）** 获取最新进展。实际检索时需结合关键词如“C21orf45 recombinant expression”、“functional studies”等。
The human C21orf45 protein, encoded by the C21orf45 gene located on chromosome 21 (21q22.11), remains poorly characterized, though it is implicated in cellular processes linked to Down syndrome pathogenesis. This gene resides in the Down Syndrome Critical Region (DSCR), a segment associated with cognitive and developmental phenotypes in trisomy 21. The protein comprises 227 amino acids, with predicted nuclear localization signals and structural motifs suggesting roles in nucleic acid binding or protein interactions.
Recombinant C21orf45 protein is typically produced using bacterial (e.g., E. coli) or mammalian expression systems, enabling functional studies and antibody development. Its low basal expression in most tissues complicates endogenous detection, making recombinant versions crucial for research. Studies associate C21orf45 with mRNA splicing regulation, cell cycle control, and oxidative stress response pathways. Knockdown experiments in cellular models reveal perturbations in proliferation and differentiation, hinting at roles in neurodevelopment.
Despite structural predictions, the precise molecular mechanisms and interactome remain undefined. Interest in recombinant C21orf45 persists due to its potential as a biomarker or therapeutic target for Down syndrome-related pathologies, particularly cognitive deficits and leukemia predisposition. Current efforts focus on crystallizing the protein and mapping post-translational modifications to unravel its biological significance.
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