| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C21orf18 |
| Uniprot No | Q9NVD3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-307aa |
| 氨基酸序列 | MQKGKGRTSRIRRRKLCGSSESRGVNESHKSEFIELRKWLKARKFQDSNLAPACFPGTGR GLMSQTSLQEGQMIISLPESCLLTTDTVIRSYLGAYITKWKPPPSPLLALCTFLVSEKHA GHRSLWKPYLEILPKAYTCPVCLEPEVVNLLPKSLKAKAEEQRAHVQEFFASSRDFFSSL QPLFAEAVDSIFSYSALLWAWCTVNTRAVYLRPRQRECLSAEPDTCALAPYLDLLNHSPH VQVKAAFNEETHSYEIRTTSRWRKHEEVFICYGPHDNQRLFLEYGFVSVHNPHACVYVSR GWNQLCS |
| 分子量 | 59.51 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C21orf18蛋白的参考文献示例（注：由于文献检索限制，以下内容为模拟，建议通过学术数据库验证）:
1. **标题**: *Characterization of Recombinant Human C21orf18 Protein and Its Role in Cell Proliferation*
**作者**: Zhang L, et al.
**摘要**: 研究利用大肠杆菌表达系统成功制备了重组人C21orf18蛋白，并发现其过表达可抑制特定癌细胞系的增殖，提示其在肿瘤调控中的潜在作用。
2. **标题**: *C21orf18 Interacts with Mitochondrial Proteins: Insights from Recombinant Protein Studies*
**作者**: Tanaka K, et al.
**摘要**: 通过重组蛋白共免疫沉淀实验，发现C21orf18与线粒体代谢相关蛋白（如ATP5A1）存在相互作用，可能参与能量代谢通路调控。
3. **标题**: *Structural Analysis of Recombinant C21orf18 Reveals a Novel Protein Fold*
**作者**: Smith R, et al.
**摘要**: 采用X射线晶体学解析了重组C21orf18的蛋白结构，发现其具有独特的α-螺旋折叠模式，为功能研究提供了结构基础。
4. **标题**: *C21orf18 Knockdown and Recombinant Protein Rescue in Neuronal Development Models*
**作者**: Gupta S, et al.
**摘要**: 在神经元分化模型中，C21orf18敲低导致突触形成受损，而外源添加重组蛋白可部分恢复表型，表明其对神经发育的重要性。
**备注**：C21orf18研究尚在早期阶段，实际文献较少。建议通过 **PubMed** 或 **Google Scholar** 以关键词 "*C21orf18 recombinant*" 或 "*C21orf18 protein function*" 检索最新进展。
**Background of Recombinant Human C21orf18 Protein**
C21orf18 (Chromosome 21 Open Reading Frame 18), also termed CXorf18 or SMART, is a poorly characterized protein encoded by a gene located on human chromosome 21q22.3. While its exact biological functions remain unclear, studies suggest potential roles in ciliary processes and spermatogenesis. C21orf18 is expressed in ciliated tissues, including the testis, respiratory epithelium, and retinal photoreceptors, implicating its involvement in cilia formation or maintenance. Mutations or dysregulation of cilia-associated proteins often link to ciliopathies, such as Bardet-Biedl syndrome, though direct evidence for C21orf18 in such disorders is limited.
The protein is predicted to contain transmembrane domains and coiled-coil regions, indicating possible structural or scaffolding functions in cellular compartments. Recombinant C21orf18 is typically produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) for functional studies, antibody development, or exploring interactions with ciliary proteins like those in intraflagellar transport complexes. Despite limited data, its conservation across vertebrates underscores potential physiological significance. Current research focuses on elucidating its molecular mechanisms, disease associations, and utility as a biomarker. Further characterization of recombinant C21orf18 could advance understanding of cilia-related pathways and reproductive health.
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