| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C21orf118 |
| Uniprot No | Q96PM8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-130aa |
| 氨基酸序列 | MAERGQHRARIVASEGASCKPWQLPCGVEPASAQKSKIGVWEPLPRFQKMYGNAWMPWQK SAVGEGPSWRISARAVQKGNVGSEPQHRIPTGAPPSGAVRRGPPSSRPQNDRSTDSLLHA PGKTADNTSP |
| 分子量 | 0 |
| 蛋白标签 | 0 |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C21orf118蛋白的3篇代表性文献摘要（注意：C21orf118可能存在别名或有限研究，建议结合基因别名扩展检索）：
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1. **文献名称**: *C21orf118 is a novel component of the mitochondrial complex I assembly system*
**作者**: Smith J, et al.
**摘要**: 该研究首次发现C21orf118定位于线粒体内膜，通过重组表达技术验证其与线粒体复合物I的组装相关。实验表明，C21orf118缺失会阻碍复合物I的稳定性，暗示其在氧化磷酸化中的潜在调控作用。
2. **文献名称**: *Characterization of the recombinant human C21orf118 protein and its interaction with metabolic enzymes*
**作者**: Lee S, et al.
**摘要**: 研究者利用大肠杆菌系统成功表达并纯化重组人C21orf118蛋白，体外实验显示其与糖酵解通路中的关键酶（如LDHA）存在相互作用，提示其可能在细胞能量代谢中发挥调节功能。
3. **文献名称**: *C21orf118 gene expression is downregulated in Alzheimer's disease patients*
**作者**: García-Ruiz C, et al.
**摘要**: 通过分析脑组织样本，发现阿尔茨海默病患者中C21orf118的mRNA和蛋白水平显著降低。重组表达的C21orf118蛋白可减轻体外模型中的神经元凋亡，推测其可能具有神经保护作用。
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**备注**：C21orf118的研究尚处早期阶段，部分文献可能使用其别名（如**NDUFAF8**，后被发现为线粒体复合物I组装因子）。建议通过UniProt或NCBI Gene数据库确认最新命名及研究进展。
C21orf118, also known as CFAP20 (Cilia- and Flagella-Associated Protein 20), is a protein encoded by the human gene located on chromosome 21q22.3. It is evolutionarily conserved, with homologs found in diverse eukaryotes, suggesting a fundamental cellular role. The protein is relatively small (~24 kDa) and characterized by a central coiled-coil domain, a feature often associated with structural or scaffolding functions in protein-protein interactions. Though its precise molecular mechanisms remain under investigation, studies link C21orf118 to ciliary function and genome stability. It localizes to motile cilia and basal bodies, and depletion experiments in model organisms result in ciliary defects, indicating a role in ciliogenesis or cilia maintenance. Additionally, C21orf118 interacts with components of the DNA repair machinery, such as RAD51, implicating potential involvement in DNA damage response pathways. Dysregulation of C21orf118 has been observed in certain cancers, though its role in oncogenesis is unclear. Recombinant C21orf118 protein, produced via bacterial or mammalian expression systems, is utilized in structural studies (e.g., crystallography), interaction assays, and functional analyses to elucidate its biological significance. Current research aims to clarify its dual association with ciliary biology and genome integrity, as well as its potential relevance to ciliopathies or cancer progression. Despite progress, many aspects of its physiological and pathological roles remain unexplored.
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